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Plasmid#126516PurposeAnion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).DepositorInsertMerMAID4
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-MerMAID7
Plasmid#126519PurposeAnion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).DepositorInsertMerMAID7
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-MerMAID1
Plasmid#126513PurposeAnion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).DepositorInsertMerMAID1
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
DH5alpha::DddI
Bacterial Strain#179631PurposeFor expression of toxic DddADepositorBacterial ResistanceGentamicinAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ma-Barnase
Plasmid#197572PurposeNegative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertsBarnase - 2xTAG
M. alvus Pyl-tRNA (6)
UseTagsnoneExpressionBacterialMutation3TAG and 45TAGPromoteraraC and lppAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ma-pREP
Plasmid#197571PurposePositive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a oriDepositorInsertsChloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
UseTagsExpressionBacterialMutation111TAG and 8TAG/112TAGPromoterAraC, T7, cat promoter (constitutive), and lpp (c…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SBECN1W
Plasmid#107937PurposeAdeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neuronsDepositorInsertmouse Beclin-1 (BECN1) (Becn1 Mouse)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP 150TAG
Plasmid#197570PurposeExpression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP 150TAG
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationN150TAGPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-Acridone-RS1 (A7)
Plasmid#197566PurposeExpression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cellsDepositorInsertsM. alvus PylRS - Acd RS1 (A7)
M. alvus Pyl-tRNA (6) (4x copies)
UseTagsNoneExpressionMammalianMutationN166A, V168G, W239CPromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Htt171-66Q-myc-WPRE
Plasmid#107934PurposeAdeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neuronsDepositorInsertExon 1 of mutant HTT, 66 polyQ repeats (HTT Human)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Htt171-18Q-myc-WPRE
Plasmid#107936PurposeAdeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neuronsDepositorInsertExon 1 of wild type HTT (HTT Human)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJ209
Plasmid#162682PurposeLidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
UseTagsHisExpressionBacterialMutationReplacement of G251-T472 with W185-F191 from PETa…PromoterT7/lacAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-CITED(216-242)+CBPTAZ1(340-439)
Plasmid#189961PurposeCoexpression of CBP TAZ1 and CITED in E coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)
Plasmid#189963PurposeCoexpression of CBP TAZ1 and CITED2 in E coliDepositorUseTagsExpressionBacterialMutationL243A, E245A, L246APromotert7Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)
Plasmid#189962PurposeExpression of CBP TAZ1 and CITED2 in E coliDepositorUseTagsExpressionBacterialMutationPromotert7Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II acceptor only control (F40-based tension sensor)
Plasmid#118719PurposeThe acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)(Y67G)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
RCAS GFP (CT#22)
Plasmid#13878DepositorInsertGFP
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
RISeGFP (CC#353)
Plasmid#16131DepositorInserteGFP
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRIAS (CC#15)
Plasmid#15147DepositorTypeEmpty backboneUseRetroviral; Avian expressionTagsgag and polExpressionMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSLIRES-11 (CC#13)
Plasmid#15145DepositorTypeEmpty backboneUseAdapter vector for cloning into prisapTagsIRESExpressionMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only