Addgene

We narrowed to 45,525 results for: ina

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pGY416

Plasmid

#166660

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

GAL1

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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alpha1Ddelta-G12-FRB

Plasmid

#87454

Purpose

rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

a1D short variants (Cacna1d Rat)

Use

Tags

FRB

Expression

Mammalian

Mutation

delete KYPAKNTTIALQMLERML

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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Sema3c(S)-AP-His

Plasmid

#72016

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf166

Plasmid

#12854

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf166

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
Sema3c(S)-Fc-His

Plasmid

#72142

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _701-902

Plasmid

#86821

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAGHa-SERBP1(189-400)

Plasmid

#172315

Purpose

Expression of the C-terminal (189-400) domain of SERBP1 in E. coli.

Depositor

David Libich

Article

Baudin et al Biomol NMR Assign. 2021 Oct;15(2):461-46

Insert

Plasminogen Activator Inhibitor 1 RNA-binding protein, residues 189-400 (SERBP1 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

deleted 1-188, 401-408

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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Sema4g-AP-His

Plasmid

#72034

Purpose

Expresses the extracellular region of the Sema4G protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4g (Sema4g Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

Plasmid

#169992

Purpose

Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

TEV-siriusGFP-FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRger1-TS-EYFP

Plasmid

#127243

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger1-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-Fc-His

Plasmid

#72068

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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(81) pcDNA3.1-BC2-Nup62-Flag-EPEA

Plasmid

#168094

Purpose

Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

Nup62-Ubc-Flag-EPEA

Use

Tags

BC2-Tag, EPEA-tag, and Flag-tag

Expression

Mammalian

Mutation

P229A, S283T on Nup62 native sequence

Promoter

T7/CMV

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-DNL-CMV-GFP

Plasmid

#83253

Purpose

Adenoviral expression of STAT5A dominant negative with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5A (Stat5a Mouse)
eGFP

Use

Adenoviral

Tags

Expression

Mutation

C-terminal deletion of 351 nucleotides (dominant …

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGY466

Plasmid

#166661

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

MET17

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A321G

Plasmid

#98670

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321G

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A321G

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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FusionRed-Sit-N-15

Plasmid

#56133

Purpose

Localization: Golgi TGN, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Sit (ST6GAL1 Human)

Use

Tags

FusionRed

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMpGWB222

Plasmid

#68613

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB219

Plasmid

#68610

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf92

Plasmid

#12780

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf92

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pUCHR-CD5Flag2-P2A-smAID-mClover

Plasmid

#110657

Purpose

Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CD5Flag2-P2A-AID-mClover

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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