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Plasmid#140239PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1DepositorInsertsgRNA targeting human MIB1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHsp70B (GB0155)
Plasmid#68172PurposeProvides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid partDepositorInsertAtHsp70B promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP HSPA14
Plasmid#19488DepositorInsertHSPA14 (HSPA14 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-POC5
Plasmid#136786PurposeMammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tagDepositorInsertSNAP-POC5 (POC5 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sm-Myocardin Omni-pShuttle
Plasmid#122122PurposeExpression of the smooth muscle version of myocardinDepositorInsertMyocardin (Myocd Mouse)
UseTagsOmniExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-17-T
Plasmid#124677PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
NELFE-mOrange pHis-//
Plasmid#53303PurposeExpresses His-tagged NELF-E-mOrange fusion protein.DepositorInsertNELF-E protein (Nelf-E Fly)
UseTagsHis and mOrangeExpressionBacterialMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJZC519
Plasmid#62278PurposeYeast dCas9-VP64 expression plasmidDepositorInsertdCas9-VP64
UseTagsHas a VP64 fusionExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJA4
Plasmid#19494DepositorInsertDNAJA4 (DNAJA4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'UAG)
Plasmid#170126PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PPP1R13L-1/1
Plasmid#91509PurposeProtein expression and purification of human SH3 domain construct PPP1R13L-1/1DepositorInsertPPP1R13L-1/1 (PPP1R13L Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-UAG site)
Plasmid#170138PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcriptDepositorInsertBoxB-RAB7A(UAG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPS0253
Plasmid#115505PurposeSuitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.DepositorInsertRlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(NC)-SH2
Plasmid#46521DepositorInsertSyk(NC) (SYK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 7-263PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHsp18.2 (GB0153)
Plasmid#68171PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A
Plasmid#115843PurposeEncodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutantDepositorInsertSIVMAC251-Vpx-Q76A
UseTags3x-FLAGExpressionMammalianMutationQ76APromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-Inp54p(D281A)
Plasmid#96813Purposephosphatase-dead control for 5HT6-YFP-Inp54pDepositorInsert5HT6-YFP-Inp54p(D281A)
UseTagsYFPExpressionMammalianMutationD281A inactivates phosphatase activityPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-258 A50I mutation
Plasmid#46271DepositorInsertVcl 1-258 (aka VD1) A50I mutation (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation1-258 (VD1 domain) A50I mutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMX GFP cdc42 Q61L
Plasmid#14568DepositorInsertcdc42 Q61L (CDC42 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationQ61LPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc NES-mCE NLS
Plasmid#82466PurposeExpresses cytoplasmic restricted form of myc tagged mRNA capping enzymeDepositorInsertNES mRNA capping enzyme (Rngtt Mouse, HIV)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only