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pRCP

Plasmid

#195862

Purpose

Encodes chromogenic protein (CP). CP inducible with rhamnose.

Depositor

Gregory Phillips

Article

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Insert

Chromogenic Protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pALPS

Plasmid

#90996

Purpose

Lentiviral vector for expression of heterologous genes

Depositor

Jeremy Luban

Article

Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Stra6L-6His

Plasmid

#174215

Purpose

Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

Depositor

Adrian Press

Article

Stafast et al ACS Appl. Polym. Mater. 2022

Insert

Stra6L (Stra6l Mouse)

Use

Tags

6-His

Expression

Mammalian

Mutation

non

Promoter

CMV

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-mRuby2

Plasmid

#126215

Purpose

Labeling of cre-expressing neurons (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mRuby2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mMCL1-WT

Plasmid

#45817

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

mMCL-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pmCellFree_KA1_ZIKV NS2A

Plasmid

#145343

Purpose

Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Feb. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-mCherry

Plasmid

#175841

Purpose

Express mCherry with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with nucleus-targeting sequence

Use

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-RaichuEV-Rac1(T17N)

Plasmid

#164902

Purpose

Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

RaichuEV-Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

T17N mutation in the Rac1 domain which make the s…

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV E

Plasmid

#145361

Purpose

Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Envelope

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHeCAHS-mEGFP-NLS

Plasmid

#205014

Purpose

The vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

Depositor

Kazuharu Arakawa

Article

Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

Insert

mEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris

Use

Tardigrade expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L S382A S468A

Plasmid

#27003

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S382A S468A

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
MSCV-Syn-PSD95.FingR-eGFP

Plasmid

#126212

Purpose

Labeling of excitatory synapses (green fluorescence) in dividing cells

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-Lyn-SpiCee

Plasmid

#140837

Purpose

mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

Lyn-SpiCee

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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hBACCS2-IRES-GFP

Plasmid

#72891

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2-IRES-GFP

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6-Smad3 WT

Plasmid

#27010

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pZ001 (AacC2c1 locus)

Plasmid

#70166

Purpose

Expresses AacC2c1 full locus

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 locus

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Sec61β

Plasmid

#174466

Purpose

Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only
pAL119

Plasmid

#21909

Depositor

Maria Castro

Article

Ali et al Mol Ther. 2004 Dec . 10(6):1071-84.

Insert

hCMV promoter expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pSO221 Phis-72 BirA GFP

Plasmid

#45100

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

BirA::GFP
unc-119 (unc-119 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

his-72 and unc-119

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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