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Plasmid#62365PurposepEF1a-puro-T2A-ATHmmuRPL10ADepositorInsertPuro-T2A-AVITEVHAmmuRPL10A
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-miRFP670nano3
Plasmid#184666PurposeHuman alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertmiRFP670nano3-human alpha-tubulin
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIREShyg-PA-GFP-alpha Tubulin
Plasmid#12296DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagsPA-GFPExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
Kras-Src FRET biosensor
Plasmid#78302PurposeFRET biosensor. To monitor Src kinase activity in mammalian cells by FRETDepositorInsertSrc biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TEVSH
Plasmid#125194PurposeHis-tagged TEV protease with solubility increasing mutationsDepositorInsertTEV_SH
UseTagsV5 His-tagExpressionBacterialMutationPromoterT7lacAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-Giantin
Plasmid#129630PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorArticleInsertmTurquoise2-Giantin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-SsAPOBEC3B
Plasmid#198536PurposeExpresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-SsAPOBEC3B
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseTagsExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL004
Plasmid#215336PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterhyper-pSpankAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3
Plasmid#165430PurposeExpresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.DepositorInsertFHS-paCaMKII(SD)
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationC450A, L514K, G528A, L531E, N538E: Light-insensit…PromoterCaMKII0.4Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-Nanobody
Plasmid#70696PurposeExpresses GST-tagged anti-mCherry Nanobody in E. coli cells.DepositorInsertmCherry-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-TetR_W43F-3xmNG
Plasmid#174880PurposePiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreenDepositorInsertTetR(W43F)-3xmNeonGreen
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-Rab6A
Plasmid#190171PurposeExpresses HaloTag-Rab6A in mammalian cells.DepositorInsertHaloTag-Rab6A
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_R248W
Plasmid#183114PurposeExpresses flag-tagged p53 with R248W mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged arginine 248 to tryptophanPromoterEF1aAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain
Bacterial Strain#99247PurposeLacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strainDepositorBacterial ResistanceTetracyclineAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
T7-Elongin C-pcDNA3
Plasmid#19998DepositorInsertElongin C (ELOC Human)
UseTagsT7-tagExpressionMammalianMutationPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-XBP1s
Plasmid#63680PurposeMammalian expression of Flag tagged XBP1sDepositorInsertXBP1 (XBP1 Human)
UseTagsFlagExpressionMammalianMutationsplicedPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-hVPS26
Plasmid#17636DepositorInsertVPS26 (VPS26A Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUAS-NanoLuc
Plasmid#87696PurposeGal4VP16 driven expression of NanoLucDepositorInsertNanoLuc
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLM-fSV2A
Plasmid#27512DepositorInsertOCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only