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Plasmid#87868PurposeExpress a active-site mutant human Atg7/Apg7 C572S in mammalian cellsDepositorInsertATG7 C572S mutant (ATG7 Human)
UseTagsExpressionMammalianMutationC572S mutantPromoterCMVAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1a-Archon2-KGC-EGFP-ER2
Plasmid#108426PurposeLentivirus production plasmid encoding for Archon2 fluorescent voltage reporterDepositorInsertArchon2-KGC-EGFP-ER2
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Archon1-EGFP
Plasmid#108417PurposeAAV production plasmid encoding for Archon1 fluorescent voltage reporterDepositorInsertArchon1-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF143-wArchon1-KGC-EGFP-ER2
Plasmid#108429PurposeCodon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmidDepositorInsertwArchon1-KGC-EGFP-ER2
UseTagsExpressionWormMutationPromoterflp-18Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP12-5ptpase enzyme
Plasmid#67515PurposeRecruitable human type IV 5-phosphatase enzymeDepositorUseTagsmRFPExpressionMammalianMutationINPP5E has C641A to destroy the C-terminal CAAX d…PromoterCMVAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-SAP97 [K64/15R]
Plasmid#128624PurposeMammalian Expression Plasmid of anti-SAP97 (Rat). Derived from hybridoma K64/15.DepositorInsertanti-SAP97 (Rat) recombinant mouse monoclonal antibody (Dlg1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP-beta-Actin-C-19
Plasmid#108412PurposeBeta-actin fused to monomeric near-infrared fluorescent protein miRFPDepositorInsertbeta-Actin
UseTagsmiRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SpRFN
Plasmid#85754PurposeExpresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).DepositorInsertCsy4-T2A-FokI-dCas9
UseCRISPRTagsCsy4 and FokI fusion via 25 amino acid (GGGGS)5 l…ExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_CH3-3
Plasmid#105853PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH3.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG1 with CH3 derived…PromoterhEF1-HTLV promAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A
Plasmid#51248PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 849,865,877,907,980,1006 mutated to A…PromoterEF1aAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5)
Plasmid#55799PurposeThis G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.DepositorInsertG protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T in alpha-s sequ…PromoterCMVAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Anti-Foxi3 [N359/28R]
Plasmid#114495PurposeMammalian Expression Plasmid of anti-Foxi3 (Mouse). Derived from hybridoma N359/28.DepositorInsertanti-Foxi3 (Mus musculus) recombinant mouse monoclonal antibody (Foxi3 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-Doc2b [N150/21.3R]
Plasmid#114516PurposeMammalian Expression Plasmid of anti-Doc2b (Rat). Derived from hybridoma N150/21.3.DepositorInsertanti-Doc2b (Rattus norvegicus) recombinant mouse monoclonal antibody (Doc2b Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF143-wArchon2-KGC-EGFP-ER2
Plasmid#108430PurposeCodon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmidDepositorInsertwArchon2-KGC-EGFP-ER2
UseTagsExpressionWormMutationPromoterflp-18Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SaRFN
Plasmid#85755PurposeExpresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).DepositorInsertCsy4-T2A-FokI-SadCas9
UseCRISPRTags3xHA tag, Csy4, and FokI fusion via 36 amino acid…ExpressionMammalianMutationD10A, N580APromoterCAGAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJC-mhc2.4kb-TALE3-VP64-P10
Plasmid#104611PurposeExpresses TALE3 under the control of a 2.4kb mhc enhancer elementDepositorInsert2.4kb mhc enhancer element (Mhc Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ289-RVR
Plasmid#138127PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoterDepositorInsertErCas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD513R, K519V and N523RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_CH2-3
Plasmid#105851PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH2.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG1 with CH2 derived…PromoterhEF1-HTLV promAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_CH1h-3
Plasmid#105850PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH1 and hinge.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG1 with CH1 and hin…PromoterhEF1-HTLV promAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only