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Plasmid#182356PurposeNuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only
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bactin:mKate2-ras
Plasmid#105963Purposetransgenesis, ubiquitous expressionDepositorInsertRas (KRAS Human)
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS
Plasmid#106355PurposeCRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locusDepositorInsertMYOD1 gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJG488
Plasmid#91196PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)DepositorInsertnTaCas9_H840A+gUbi8+gUbi1+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA13_G-alpha
Plasmid#109913PurposeProtein expression and purification of GNA13_G-alphaDepositorInsertGNA13_G-alpha (GNA13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
STIMATE(1-240)
Plasmid#71280PurposeExpresses STIMATE or TMEM110 (1-240) in mammalian cellsDepositorInsertSTIMATE (1-240) (TMEM110 Human)
UseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2519
Plasmid#91088PurposeModule C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterOsU3Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lyn-CFP-GAI(1-151)
Plasmid#37312DepositorInsertGAI (GAI Mustard Weed)
UseTagsCFP and LynExpressionMammalianMutationcontains aa 1-151PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP
Plasmid#127363PurposeBinary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
UseTagsGS linker, NES, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
WNT11-bio-His
Plasmid#53430PurposeExpresses full-length Protein Wnt-11 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertWNT11 (WNT11 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJS278
Plasmid#134139PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255, R155APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GTF2F1_TFIIF
Plasmid#110037PurposeProtein expression and purification of GTF2F1_TFIIFDepositorInsertGTF2F1_TFIIF (GTF2F1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS2131
Plasmid#111895PurposessAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPREDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Lyn-CFP-GAI(1-532)
Plasmid#37314DepositorInsertGAI (GAI Mustard Weed)
UseTagsCFP and LynExpressionMammalianMutationcontains aa 1-532PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSR06
Plasmid#69152Purposeread-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr IIDepositorInsertsmCherry
eGFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0 and codon-optimzed index…Promoterrps-27Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G5
Plasmid#86618PurposeExpresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1DepositorInsertATP1A1 G5 crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via hdr using ouabainTags3xHAExpressionMammalianMutationPromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-1-173
Plasmid#104193PurposeExpresses human AR aa 1-173 in pGEX-5x-1 bacterial expression vector with GSTDepositorInserthuman androgen receptor aa 1-173 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS1964
Plasmid#82574PurposeHigh copy number plasmid containing Ple305 MiniPromoter insert.DepositorInsertPle305
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_NR0B1_Hormone-recep
Plasmid#109835PurposeProtein expression and purification of NR0B1_Hormone-recepDepositorInsertNR0B1_Hormone-recep (NR0B1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only