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CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

Plasmid

#203909

Purpose

Encodes wild type Gal4-VP64 transcription factor in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4-VP64-IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(wt)

Plasmid

#13719

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(wt) (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NDP52

Plasmid

#135296

Purpose

stably express GFP-tagged NDP52 for CID induced mitophagy

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

NDP52 (CALCOCO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-ArchT-tdTomato

Plasmid

#28305

Purpose

Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV5

Insert

ArchT-tdTomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdTomato

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-CC

Plasmid

#92127

Purpose

Expression of codon-optimized Cas9 with Pcbh1 promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9

Use

CRISPR; Trichoderma reesei expression

Tags

Expression

Mutation

Promoter

Pcbh1

Available since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-XZ066-EGFRvIII

Plasmid

#20737

Depositor

Alonzo Ross

Article

Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Insert

EGFRvIII (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

EGFR with a 267 amino acid deletion in the extrac…

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-FOXA1

Plasmid

#153109

Purpose

Expression of Flag tagged human FOXA1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXA1 (FOXA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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MAC3-tag-C

Plasmid

#185481

Purpose

Gateway compatible destination vectors containing StrepIII/HA/UltralD

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Type

Empty backbone

Use

Tags

StrepIII-HA-UltraID

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHCMV_DHOV_GP

Plasmid

#207267

Purpose

Mammalian expression of Dhori virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DHOV_GP

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-CAAX

Plasmid

#86056

Purpose

A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Homo sapiens Harvey rat sarcoma (HRAS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains the region from 825-857 of HRAS as a far…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NE1m (AAV Retrograde)

Viral Prep

#123308-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-GRAB_NE1m (#123308). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NE1m plasmid DNA. Synapsin-driven expression of GRAB-NE1 norepinephrine sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Yulong Li

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Promoter

Syn

Tags

None

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only
pUCmini-iCAP-AAV.MaCPNS1

Plasmid

#185136

Purpose

non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

Insert

Synthetic construct isolate AAV-MaCPNS1 VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-BRD2

Plasmid

#65376

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD2 (BRD2 Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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FUG-Pten

Plasmid

#140732

Purpose

Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-Pten (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

hUbiC

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSE100

Plasmid

#17972

Depositor

Sabine Ehrt

Article

Guo et al J Bacteriol. 2007 Jul . 189(13):4614-23.

Insert

pmyc1tetO

Use

Mycobacteria expression

Tags

Expression

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pVAX1-BMP2

Plasmid

#137909

Purpose

Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Insert

BMP2 (BMP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

Plasmid

#162381

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

CAG

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-VCPIP1

Plasmid

#85671

Purpose

Expression of VCPIP1 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

VCPIP1 (VCPIP1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-SYP1-miniSOG-T2A-mCherry

Plasmid

#50972

Purpose

AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)

Use

AAV

Tags

T2A-mCherry and miniSOG

Expression

Mutation

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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