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Plasmid#32402DepositorTypeEmpty backboneUseTagsbiotinylation peptide and ratCd4d3+4ExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only
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phage - ubc - nls - mcp - egfp - BirA*
Plasmid#131134PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-MCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-miniTurbo-V5-STOP_IDG-K
Plasmid#158158PurposeGateway destination clone of miniTurbo-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-miniTurbo-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRDX1A-c001
Plasmid#175473PurposeProtein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.DepositorInsertPRDX1 (PRDX1 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-BioID-STOP_IDG-K
Plasmid#158163PurposeGateway destination clone of BioID (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-BioID
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - mCherry - BirA*
Plasmid#131136PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-mCherry-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-hcRed-V5-TurboID_IDG-K
Plasmid#135238PurposeGateway destination clone of hcRed (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInserthcRed-V5-TurboID
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-dsRed_IDG-K
Plasmid#135260PurposeGateway destination clone of dsRed (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-dsRed
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRDX6A-c001
Plasmid#175474PurposeProtein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.DepositorInsertPRDX6 (PRDX6 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationSee Depositor Comments BelowPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - cMycBirA*
Plasmid#131133PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-cMycBirA*
UseLentiviralTagsNLS, HA, cMyc (N-terminal of BirA*)ExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K
Plasmid#158160PurposeGateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-3xHA-miniTurbo-3XNLS
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-TLK2-V5_IDG-K
Plasmid#135251PurposeGateway destination clone of TLK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTLK2-V5 (TLK2 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-2xV5-miniTurbo-NES-STOP_IDG-K
Plasmid#158161PurposeGateway destination clone of miniTurbo-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsert2xV5-miniTurbo-NES
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-RIOK2_IDG-K
Plasmid#170586PurposeGateway destination clone of RIOK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-RIOK2 (RIOK2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
K560_AviN_coBirA
Plasmid#129766PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-TcRb:mCherry-FKBP
Plasmid#72904PurposeER-targeted mCherry-tagged recruiter constructDepositorInsertT cell receptor β chain
UseLentiviralTagsbiotinylation acceptor peptide (AP) and mCherry, …ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-NEK7-V5-miniTurbo_IDG-K
Plasmid#189882PurposeGateway destination clone of NEK7 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertNEK7 (NEK7 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NEK5_IDG-K
Plasmid#189876PurposeGateway destination clone of NEK5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertNEK5 (NEK5 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-2xV5-TurboID-NES-STOP_IDG-K
Plasmid#158162PurposeGateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsert2xV5-TurboID-NES
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only