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Plasmid#167529PurposeTo increase the length of the linker between Venus and Noxa-4E proteinsDepositorInsertPMA-induced protein 1 (PMAIP1 Human)
UseTagsVenusExpressionMammalianMutationC25E, L29E, F32E, L36EPromoterCMVAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-13aa-Venus-pEGFP-C3
Plasmid#166753PurposeFor expression of mCerulean3 linked to Venus by 13 amino acid linkerDepositorInsertmCerulean3-13aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-79aa-ActA-pEGFP-C1
Plasmid#166763PurposeTo target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.DepositorInsertActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Noxa)-pEGFP-C1
Plasmid#166761PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of NoxaDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Noxa…PromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-39aa-Venus-pEGFP-C3
Plasmid#166755PurposeFor expression of mCerulean3 linked to Venus by 39 amino acid linkerDepositorInsertmCerulean3-39aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (E12,14A)-mVenus
Plasmid#168499PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9R)-mVenus
Plasmid#168498PurposeMammalian expression of the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (E12,14K)-mVenus
Plasmid#168488PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9E)-mVenus
Plasmid#168487PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K22,24A)-mVenus
Plasmid#168484PurposeMammalian expression of the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9A)-mVenus
Plasmid#168483PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (G2A)-mVenus
Plasmid#168482PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-31aa-Venus-pEGFP-C3
Plasmid#166754PurposeFor expression of mCerulean3 linked to Venus by 31 amino acid linkerDepositorInsertmCerulean3-31aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Bik)-pEGFP-C1
Plasmid#166760PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BikDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Bik …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Bid)-pEGFP-C1
Plasmid#166759PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BidDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of mouse Bid …PromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Bad)-pEGFP-C1
Plasmid#166758PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BadDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Bad …PromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim-DC2-H2B2-Venus
Plasmid#129556PurposepClimDC-H2B:mV nuclear markerDepositorInsertHistone 2B fused to venus
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-mVenus-P2A-PuroR-HA
Plasmid#163345PurposeRetroviral vector to express cytosolic mVenus and an HA-tagged puromycin selection markerDepositorInsertmVenus
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG
Plasmid#139963PurposeOver-expression of native 3-hydroxy-3-methyl glutaryl CoA reductaseDepositorInsertConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG
Plasmid#139964PurposeOver-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductaseDepositorInsertConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only