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Plasmid#178883PurposeVector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.DepositorInsertU6-Nla repeat-BbsI-Nla repeat
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75S)
Plasmid#137828PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75S)
UseTags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Serine in NTEVp fragmentPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
A3Bi-Cas9n-UGI
Plasmid#119135PurposeBase editor made from full-length APOBEC3B with an L1 intron to prevent self-restrictionDepositorInsertAPOBEC3B L1 intron-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationL1 intron insertionPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Sa-SauriCas9
Plasmid#135967PurposeExpresses Sa-SauriCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-PPxY
Plasmid#61006PurposeExpresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 PPxY mutation (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationPPxY mutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH7
Plasmid#83554PurposeUsed to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.DepositorInsertADH2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterADH2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-42
Plasmid#172758PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-42
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-mPRMT7-GFP
Plasmid#87104PurposeExpress mouse PRMT7 with Flag-tag in mammalian cellsDepositorInsertProtein arginine methyltransferase 7 (Prmt7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterEF1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-18
Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PDZD7-TwinStrepII
Plasmid#83198PurposeExpression of tagged protein in mammalian cellsDepositorInsertPDZD7 (Pdzd7 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-FKBP
Plasmid#20220DepositorInsertFKBP (FKBP1A Human)
UseTagsHA and VhExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS
Plasmid#199350PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOstarExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP
Plasmid#199361PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOstar-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP
Plasmid#199362PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOstar-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAV1-deltaN-mEGFP
Plasmid#27707DepositorInsertcaveolin-1, N-terminal truncated (60-178) (CAV1 Canis lupus familiaris)
UseTagsmonomeric EGFPExpressionMammalianMutationN-terminal truncation with AA 1-59 missing. The r…PromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST EIF2C3
Plasmid#19889DepositorInsertEukaryotic translation initiation factor 2C, 3 (AGO3 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-2A-rtTA-TcVA
Plasmid#24592DepositorInsertGateway(TM) cassette
UseLentiviralTagsVAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PACSIN2-1/1
Plasmid#91330PurposeProtein expression and purification of human SH3 domain construct PACSIN2-1/1DepositorInsertPACSIN2-1/1 (PACSIN2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1 R44Q-14-mVenusC
Plasmid#191394PurposeR44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenusDepositorInsertpcDNA3 Flag-MBD1 R44Q-14-mVenusC
UseTags3xFLAGExpressionMammalianMutationR44QPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-del-N
Plasmid#61004PurposeExpresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 delete N (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationdeleted amino acids 17-310PromoterCMVAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only