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pDONR223_IDH1_p.R132C

Plasmid

#81726

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R132C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.R132C

Plasmid

#81726

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R132C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBA562

Plasmid

#186237

Purpose

pTet-RfxCas13d crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HO-pGAL-poly-KanMX4-HO

Plasmid

#51664

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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HO-pGAL-poly-KanMX4-HO

Plasmid

#51664

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-4

Plasmid

#72679

Purpose

Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.

Depositor

Csaba Pál

Article

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Use

Tags

none

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-4

Plasmid

#72679

Purpose

Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.

Depositor

Csaba Pál

Article

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Use

Tags

none

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-GFP-Tmem192

Plasmid

#134630

Purpose

lysosomal marker

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-GFP-Tmem192

Plasmid

#134630

Purpose

lysosomal marker

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1_WT_OCT4

Plasmid

#40633

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Use

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1_WT_OCT4

Plasmid

#40633

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Use

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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mEmerald-Vinculin-N-21

Plasmid

#54304

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-Vinculin-N-21

Plasmid

#54304

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-4T-1-3xFLAG-HSP27

Plasmid

#129229

Purpose

Encodes FLAG tagged human HSP27 for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

HSP27 (HSPB1 Human)

Use

Tags

FLAG and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xFLAG-HSP27

Plasmid

#129229

Purpose

Encodes FLAG tagged human HSP27 for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

HSP27 (HSPB1 Human)

Use

Tags

FLAG and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pStrep-1xEGFP-N1

Plasmid

#122488

Purpose

Expression of Strep-tagged 1xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 1xEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pStrep-1xEGFP-N1

Plasmid

#122488

Purpose

Expression of Strep-tagged 1xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 1xEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET empty polycistronic destination vector (2D)

Plasmid

#29774

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET empty polycistronic destination vector (2D)

Plasmid

#29774

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-pro-sumo1

Plasmid

#25101

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Changed Serine 2 to Alanine

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only