Addgene

We narrowed to 24,706 results for: sta

Showing: 7321 - 7340 of 24706 results

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pETM-60-NcQDE2 _506-938-H609W_M

Plasmid

#146965

Purpose

Bacterial Expression of NcQde-2_506-938-H609W

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-H609W

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-K634A_M

Plasmid

#146966

Purpose

Bacterial Expression of NcQde-2_506-938-K634A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-K634A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-R895A_M

Plasmid

#146967

Purpose

Bacterial Expression of NcQde-2_506-938-R895A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-R895A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-Y595L_M

Plasmid

#146968

Purpose

Bacterial Expression of NcQde-2_506-938-Y595L

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-Y595L

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-miR21mutant-SunTag-TREAT

Plasmid

#196933

Purpose

SunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-2 σ2

Plasmid

#198345

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-2 σ2

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

R42G substitution, and silent substitutions in co…

Promoter

ADH1

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-1 σ1C

Plasmid

#198336

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1C

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

S148C substitution

Promoter

ADH1

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-AP-3 σ3A

Plasmid

#198347

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-3 σ3A

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-AP-3 σ3B

Plasmid

#198348

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-3 σ3B

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-let7-SunTag-TREAT

Plasmid

#196926

Purpose

SunTag cassette was added in the coding region in the let7-IRE-TREAT reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
IRE-let7R-SunTag-TREAT

Plasmid

#196927

Purpose

SunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80l AEC

Plasmid

#196473

Purpose

Protein expression of SpyTag-ELP-CarHC

Depositor

Fei Sun

Article

Yang et al Matter (2020) 2, 233–249

Insert

AEC

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003_sgTFAP4 guide 1

Plasmid

#193450

Purpose

ko TFAP4 guide 1

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

Tfap4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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MON

Plasmid

#187420

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1
ATP1B1

Use

Tags

Expression

Insect

Mutation

Promoter

PH and p10

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIRSTD)-mEos3.2

Plasmid

#189719

Purpose

titratable expression of mEos3.2 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mEos3.2

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD/HisB

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPEPT1

Plasmid

#195001

Purpose

Production of peptides in E. coli as GB1 fusion

Depositor

Marko Hyvönen

Article

Pantelejevs et al Chem Sci. 2023 Nov 21;14(47):13915-13923

Type

Empty backbone

Use

Tags

His8-tag and StrepTagII-GB1

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna2

Plasmid

#192796

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna2

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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