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Plasmid#162279PurposeEukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN2A Variant 1, stabilized (SCN2A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp4551.PromoterCMVAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_PLD1
Plasmid#45268DepositorInsertPLD1 (PLD1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pGEX Stat6-SH2
Plasmid#46517DepositorInsertStat6 (STAT6 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 510-654PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Short
Plasmid#164121PurposeDetect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Short
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187948PurposeER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertER50-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianMutation6 mutations, T371A, L384M, M421G, G521R, Y537S, N…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Long
Plasmid#164120PurposeDetect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Long
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_TLOC1 (399aa)
Plasmid#45288DepositorInsertTLOC1 (SEC62 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-mutKIR-SQSTM1
Plasmid#223719PurposeExpresses FLAG-TurboID-mutKIR-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationMutation 347-DPSTGE-352 to 347-APSAAA-352PromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1
Plasmid#223716PurposeExpresses FLAG-TurboID-dPB1-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationdeletion 1-119PromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1
Plasmid#223717PurposeExpresses FLAG-TurboID-dUBA-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationdeletion 384-Stop codonPromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-mutLIR-SQSTM1
Plasmid#223718PurposeExpresses FLAG-TurboID-mutLIR-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationMutation 335-DDDW-338 to 335-AAAA-338PromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS31-cCARLIN
Plasmid#160928PurposeContains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)DepositorInsertCARLIN gRNAs and CAG-GFP-CARLIN array
UseMouse TargetingTagsExpressionMammalianMutationPromoterU6 promoter for gRNAs; CAG promoter for GFPAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTRY-GFP
Plasmid#15301DepositorInserthrGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSC101-Donor
Plasmid#140630PurposeKanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.DepositorInsertLE, RE, KanR, temperature sensitive pSC101 origin of replication
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
Plasmid#162280PurposeEukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN8A Variant 1, stabilized (SCN8A Human)
UseTagsIRES-mScarletExpressionMammalianMutationModified human HSPA5 intron 4 inserted after bp23…PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5/DEST-VPS35
Plasmid#21691DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseLentiviral; Invitrogen gateway destination vectorTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaPylRS
Plasmid#200225PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
UseTagsnoneExpressionBacterialMutationnonePromoterproK-lacO and tacIAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-195-3p
Plasmid#103311PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-195-3p target (MIR195 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only