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Plasmid#71978PurposeExpresses the entire Netrin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNtn1 (Ntn1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-MfPylRS (human-opti)
Plasmid#164081PurposeTo express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansionDepositorInsertMfPylRS(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-Pom121-mScarlet
Plasmid#171491PurposeT7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNADepositorInsertPom121 (Pom121 Mouse)
UsePgehmeTagsmScarletExpressionMutationPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK TT 3xFLAG SMAD4 neo
Plasmid#83273PurposeLentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tagDepositorInsertSMAD4 (SMAD4 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
4LNDDE488Q-pCDNA3.1(+)
Plasmid#172228PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
UseTags6xHis and FlagExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-RARS-5
Plasmid#166535PurposescFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-KARS-5
Plasmid#166530PurposescFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-DARS-1
Plasmid#166538PurposescFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-LARS-15
Plasmid#166542PurposescFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
ALIX(1-702)-mNeonGreen
Plasmid#191193PurposeTruncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.DepositorInsertALIX (1-702) (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationDeletion of ALIX C-terminal PRD.PromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hHP1BP3-HA
Plasmid#165468PurposeHuman HP1BP3 mammalian expression vector with C-terminal HA tag.DepositorInsertHeterochromatin protein 1 binding protein 3 (HP1BP3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-BECN1
Plasmid#99328PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorInsertBeclin 1 (BECN1 Human)
UseTagsStrep-Strep-FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcw107
Plasmid#64648Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV sMTase (eGFP)
Plasmid#89930PurposeExpresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.DepositorInsertdCas9-MC-IRES-MN
UseTagsFlag and HAExpressionMammalianMutationdeactivated Cas9PromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 K70M
Plasmid#52973Purposeinsect expression of SIK3 K70M (kinase dead mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationK70M kinase dead mutantPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-RIAM(1-666)
Plasmid#80028PurposeMammalian expression of GFP-RIAM(1-666)DepositorInsertRIAM (APBB1IP Human)
UseTagsEGFPExpressionMammalianMutationaa 1-666PromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(2xD-E)DsRed
Plasmid#200111PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertmodified EGR1 promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter2 copies of D-E element, no other promoter elemen…Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-mCasp8
Plasmid#208782PurposeInducibly expresses mouse caspase8 fused to light-activatable Cry2DepositorInsertmCherry mCaspase8 Cry2 (Casp8 Mouse)
UseLentiviralTagsCry2 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLXSN-FLK1 TM
Plasmid#65249PurposeExpression of dominant negative FLK1 in mammalian cellsDepositorInsertFLK1 TM (Kdr Mouse)
UseRetroviralTagsExpressionMammalianMutationdeleted AA 786-1346PromoterLTRAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only