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Plasmid#160735PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in LT)
Plasmid#70033PurposepUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding regionDepositorInsertTSPyV full genome, linearized in Large T-antigen
UseCloning vectorTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
CaV2.1 (pcDNA6)
Plasmid#26578DepositorInsertCacna1a (Cacna1a Mouse)
UseTagsHis6 and V5ExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
hSERT pcDNA3
Plasmid#15483DepositorInserthSERT (SLC6A4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-ARPC3
Plasmid#31580DepositorInsertActin related protein 2/3 complex, subunit 3 (ARPC3 Human)
UseTagsHis and StrepII-tagExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
retro-gRNA-mRFP1
Plasmid#112914PurposeRetrovirus expressing mRFP1 with BBSI cloning sites for sgRNADepositorInsertmRFP1
UseRetroviralTagsExpressionMutationPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
epi-ABEmax-NG
Plasmid#135976PurposeExpresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
a1Ic-HE3-pcDNA3
Plasmid#45810DepositorInsertCACNA1I (CACNA1I Human)
UseMaking crna for oocyte injectionsTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version1
Plasmid#197931PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTagsFLAG tag and SV40 NLS and nucleoplasmin NLSExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal.Sth.dCas9
Plasmid#171088PurposeCRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.DepositorInsertsToxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
DHFR-TSc3
UseToxoplasma gondii expressionTagsExpressionMutationNote: A S245F mutation is present but did not app…PromoterDHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
UKNP6.1.1
Plasmid#98386PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
UseTagsExpressionBacterialMutationPromoterSpectinomycin resistance gene promoter and noAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV1524
Plasmid#111431PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB11 (pDEST-DHFR F[1,2] C-term, NatR)
Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
UseTagsExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPA8
Plasmid#19541DepositorInsertheat shock 70kDa protein 8 (HSPA8 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BNRF1
Plasmid#198580PurposeGateway Entry CloneDepositorInsertBNRF1 (BNRF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationNone (wildtype)PromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_rtTA-P2A-BFP_HygroR
Plasmid#186738PurposertTA-P2A-BFP expression construct cloned into lentiviral backboneDepositorInsertrtTA
UseLentiviralTagsP2A-BFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only