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Plasmid#51405PurposeRetroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)DepositorInsertSka2 IMAGE 4685972 (SKA2 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIPR-bio-His
Plasmid#50824PurposeExpresses enzymatically monobiotinylated full length RIPRDepositorInsertRIPR
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJB8
Plasmid#19531DepositorInsertDnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAIP Nup62Fv2GFP
Plasmid#139684PurposeNup62 dimerization construct fused to 2 copies of GFP under an SFFV promoterDepositorInsertNup62 (NUP62 Human)
UseLentiviralTags2 copies of GFP and Dimerization domain FKBPExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBLO 64.1
Plasmid#123120PurposeE. coli expression vector for active deltaproteobacteria CasXDepositorInsertCasX
UseCRISPRTagsExpressionMutationPromoterNAAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)
Plasmid#136646PurposeExpression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1R312A (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscALPS gag-gfp/vpx
Plasmid#115808PurposeSFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpxDepositorInsertgag-gfp
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgocatdead
Plasmid#210322PurposeBacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e(TadA V106W)
Plasmid#138509PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-nSaCas9
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
RS18960_pMQ30_KO_Kan
Plasmid#185393PurposeNon-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11DepositorInsertsRR42_RS18960 upstream flanking homology region
RR42_RS18960 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-PPHLN1 ts1
Plasmid#115877PurposePPHLN1 knockdownDepositorInsertPPHLN1 shRNA (PPHLN1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
Plasmid#73404PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rab8-T22N(Flag) in pcDNA3.1neo
Plasmid#46784Purposeexpression of T22N Rab8a in mammalian cellsDepositorInsertRAB8A T22N (RAB8A Human)
UseTagsFLAGExpressionMammalianMutationT22N dominant negativePromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV14-mULK1
Plasmid#24301DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG metap2
Plasmid#32004DepositorInsertmetap2 (Metap2 )
UseTagsFLAGExpressionMammalianMutationPromoterCMV, SP6Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB4
Plasmid#19499DepositorInsertDNAJB4 (DNAJB4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO)3
Plasmid#127150PurposeMammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsert3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SNX18-1/1
Plasmid#91369PurposeProtein expression and purification of human SH3 domain construct SNX18-1/1DepositorInsertSNX18-1/1 (SNX18 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only