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Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMVblast_SLC38A2_wt
Plasmid#156180PurposeCMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.DepositorInsertSLC38A2 (SLC38A2 Human)
UseTagsExpressionMammalianMutationSilent mutations to prevent targeting by sgRNA se…PromoterCMVAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Snap-Omp25
Plasmid#69599PurposeSnap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondriaDepositorInsertSnap-Omp25
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF0040
Plasmid#143684PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF676 (ZNF676 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCU-PDC1
Plasmid#45323DepositorTypeEmpty backboneUseC. glabrata expressionTagsExpressionMutationPromoterPDC1Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
HP17_KO7
Plasmid#120346PurposeHelper plasmid for production of phagemid ssDNA, based on M13KO7DepositorInsertCoding part of the M13KO7 helper phage genome
UseTagsExpressionBacterialMutationPromoternative phage promotersAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mef2A-shRNA
Plasmid#32899DepositorInsertMef2a (Mef2a Rat)
UseRNAiTagsExpressionMammalianMutationPromoterH1-RNAAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS2241
Plasmid#111896PurposessAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle320 (POU4F1 Human)
UseAAVTagsExpressionMutationPromoterPle320 (POU4F1 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MCP-M-MLV RT∆RNase H
Plasmid#213749PurposeFor circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cellsDepositorInsertMCP-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-mCh-CLC
Plasmid#60035PurposeExpresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chainDepositorInsertCRY2-mCh-Clathrin Light Chain (CRY2 Mouse, Mustard Weed)
UseTagsExpressionMammalianMutationM354I, E490G CRY2, A9D*PromoterCMVAvailable sinceOct. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c4X
Plasmid#75288PurposeExpresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneUseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceJuly 19, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWUR702
Plasmid#53079PurposeInducible TtAgo expressionDepositorInsertTtAgo
UseTagsStrep-II tagExpressionBacterialMutationPromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-FLPo-T2A-GFP
Plasmid#161766Purposeexpress FLPo and GFP under the control of human synapsin promoterDepositorInsertsFLPo
T2AEGFP
UseAAVTagsExpressionMammalianMutationPromoterhSynapsin1Available sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-Foxo1ADA (pCMV5)
Plasmid#12143DepositorInsertFoxo1 ADA (Foxo1 Mouse)
UseTagsHA and mycExpressionMammalianMutationPhosphorylation-deficient, also referred to as co…PromoterCMVAvailable sinceAug. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-Foxo1ADA (pCMV5)
Plasmid#12143DepositorInsertFoxo1 ADA (Foxo1 Mouse)
UseTagsHA and mycExpressionMammalianMutationPhosphorylation-deficient, also referred to as co…PromoterCMVAvailable sinceAug. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTHMT_hnRNPF_FL
Plasmid#139114Purposeexpress FL hnRNPF with N-terminal maltose binding protein tagDepositorInserthnRNPF (HNRNPF Human)
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpliceExpress
Plasmid#32485DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNitET-SacB-kan
Plasmid#107692PurposeExpression of the phage Che9c RecET system for Recombineering in mycobacteriaDepositorInsertsChe9 phage RecET
PNit2-NitR
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2E2-HA-mEGFP
Plasmid#129595PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only