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Plasmid#184952PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cluc-RMA
Plasmid#189624PurposeExpresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.DepositorInsertCypridina luciferase fused to Fc
UseAAV and LuciferaseTagsCluc and IgG1 FcExpressionMammalianMutationPromoterhSynAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Plxnd1-Fc-His
Plasmid#72132PurposeExpresses the extracellular region of the PlexinD1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnd1 (Plxnd1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-Tpr
Plasmid#60882PurposeExpresses Flag-Tagged nucleoporin Tpr in Mammalian CellsDepositorInsertTpr (TPR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC119
Plasmid#118884Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease and mGFP, cl…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC841 - pAAV minP secNLuc
Plasmid#82498PurposeLongitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivoDepositorInsertsecNLuc
UseAAVTagsExpressionMammalianMutationPromoterc-Fos minimal promoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sema3c(L)-AP-His
Plasmid#72015PurposeExpresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3c (Sema3c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SNAP-CRY2-VHH(GFP)
Plasmid#58370PurposeFor use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MPDepositorInsertCRY2[1-498] (CRY2 Mustard Weed)
UseTagsSNAP and vhhGFP4ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EFS-FlpO
Plasmid#191509PurposeLentiviral Flp Recombinase driven by the EFS promoterDepositorInsertflp recombinase (flp )
UseLentiviralTagsExpressionMammalianMutationPromoterEF1a/EFS promoter insertedAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO(ChRger2-TS-YFP)
Plasmid#127239PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSA1
Plasmid#52261PurposeBacterial expression of human 6His-tagged SUMO1 and SUMO-E1DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-TRPM1 cDNA
Plasmid#29782DepositorInsertTRPM1 (TRPM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
(318) pcDNA3.1-myc-OGA(1-400)D174N
Plasmid#168096PurposeMyc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutationDepositorInsertmyc-OGA(1-400, D174N)
UseTagsmycExpressionMammalianMutationD174N catalytically inactive OGAPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.1-AP-His
Plasmid#72042PurposeExpresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6d.1 (Sema6d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b_RanT24N
Plasmid#186271PurposeExpression vector of His-RanT24N in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
UseTagsHis tagged and His-tagged at N terminalExpressionBacterialMutationChanged T24 into NPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB122
Plasmid#68576PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
RH5-bio
Plasmid#47780PurposeExpresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RH5
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
CB6-GFP-TAOK2 (K57A)
Plasmid#197114PurposeExpression of kinase-deficient GFP-tagged TAOK2 (K57A) / PSK1 (K57A) in mammalian cellsDepositorInsertTAOK2 (K57A) (TAOK2 Human)
UseTagsGFPExpressionMammalianMutationChanged K 57 to APromoterCMVAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TIMP1-bio-His
Plasmid#52089PurposeExpresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTIMP1 (TIMP1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sema6c-Fc-His
Plasmid#72167PurposeExpresses the extracellular region of the Sema6C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema6c (Sema6c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only