Addgene

We narrowed to 29,354 results for: uros

Showing: 7241 - 7260 of 29354 results

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FUS_LC_charge_RtoK

Plasmid

#139127

Purpose

express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Add K and D to FUS LC so it has the same charge p…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Bcan-Ntrk1_4

Plasmid

#136413

Purpose

Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Myb-Qk_1

Plasmid

#136415

Purpose

Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Myb)_U6_sgRNA(Qk)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Fyn-cGASΔN-HA

Plasmid

#130922

Purpose

Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

Fyn-ΔNcGAS

Use

Tags

HA

Expression

Mammalian

Mutation

cGAS N terminus (aa1-159) replaced with the dual …

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL405 CHYRON1 integration construct

Plasmid

#126442

Purpose

To integrate the CHYRON1 locus at HEK293site3.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON1 hgRNA
pCMV-puro

Use

Tags

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD52-bglpA

Plasmid

#89704

Purpose

Human CD52 expression plasmid with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CD52-bglpA (CD52 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF12

Plasmid

#105593

Purpose

retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

silent mutations to make it resistant to the targ…

Promoter

MSCV-LTR

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF12(50-130)

Plasmid

#105595

Purpose

retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

truncation fragments containing aa (50-130), and …

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PRKCG gRNA (BRDN0001147740)

Plasmid

#76898

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCG

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCG (PRKCG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCG gRNA (BRDN0001146492)

Plasmid

#76899

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCG

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCG (PRKCG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCG gRNA (BRDN0001146495)

Plasmid

#76900

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCG

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCG (PRKCG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAR1B gRNA (BRDN0001146606)

Plasmid

#76838

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1B (PRKAR1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAR1B gRNA (BRDN0001144765)

Plasmid

#76839

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1B (PRKAR1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAR1B gRNA (BRDN0001147089)

Plasmid

#76840

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1B (PRKAR1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PANK2 gRNA (BRDN0001162474)

Plasmid

#76810

Purpose

3rd generation lentiviral gRNA plasmid targeting human PANK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PANK2 (PANK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PANK2 gRNA (BRDN0001162362)

Plasmid

#76811

Purpose

3rd generation lentiviral gRNA plasmid targeting human PANK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PANK2 (PANK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PANK2 gRNA (BRDN0001144887)

Plasmid

#76812

Purpose

3rd generation lentiviral gRNA plasmid targeting human PANK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PANK2 (PANK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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mGDF8 donor CFP-hDF

Plasmid

#72590

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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hGDF8 donor CFP-hDF

Plasmid

#72591

Purpose

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pX68

Plasmid

#46857

Purpose

OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 A138V (TP53 Mouse, Human)

Use

Tags

Expression

Mammalian

Mutation

p53 A138V

Promoter

Available since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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