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Plasmid#80325PurposeExpresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tagsDepositorInsertPXDNL-003 (PXDNL Human)
UseTagsBiotinExpressionMammalianMutationH170A and H256APromoterCMVAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)
Plasmid#70195PurposePost cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vectorDepositorInserttetR-deGFP fusion
UseTagsExpressionMutationPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-mOC-STAMP (N162D)-GFP
Plasmid#73580PurposeLentivirus expression of mOC-STAMP with N162D mutationDepositorInsertOC-STAMP (Ocstamp Mouse)
UseLentiviralTagsGFPExpressionMutationchanged asparagin (N) 162 to Aspartic Acid (D)PromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
MT79
Plasmid#63112PurposeThe artificial microRNA is designed for down-regulating expression of ACD6 mRNA.DepositorInsertamiR-ACD6
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1412
Plasmid#63787PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection markerDepositorInserteCitrine-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cs2+Etv4 S100D
Plasmid#49094Purposeexpression of Etv4 S100D mutantDepositorInsertEtv4 S100D (etv4 Zebrafish)
UseXenopus/avian/zebrafishTagsExpressionMammalianMutationS100DPromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-F112S
Plasmid#48727PurposeBacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationPhenylalanine 12 to SerinePromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-L108S
Plasmid#48725PurposeBacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationLeucine 108 to SerinePromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-I98S
Plasmid#48723PurposeBacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationIsoleucine 98 to SerinePromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-I73S
Plasmid#48722PurposeBacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationIsoleucine 73 to SerinePromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-V69S
Plasmid#48721PurposeBacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationValine 69 to SerinePromoterT7Available sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_hcRED
Plasmid#45286DepositorInserthcRed
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJune 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWzl_neo_DEST_flag_hcRED
Plasmid#45458DepositorInserthcRED
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta 6X mutant
Plasmid#128816PurposeTo make virus expressing Doc2beta 6X mutantDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationD163A, D218A, D220N, D303A, D357A, D359APromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.2-RBD
Plasmid#184830PurposeMammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
UseTags6X His tagExpressionMammalianMutationSARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…PromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.1-RBD
Plasmid#184831PurposeMammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
UseTags6X His tagExpressionMammalianMutationSARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…PromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(CAT)T7-SB100
Plasmid#34879DepositorInsertSB100X transposase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
UseTagsExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…PromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only