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Showing: 7241 - 7260 of 10634 results

  3. SORT1-bio-His

    Plasmid
    #52024
    Purpose
    Expresses full-length Sortilin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SORT1 (SORT1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52024-plasmid-map-sequence-id-80096

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  4. TMEM-bio-His

    Plasmid
    #51856
    Purpose
    Expresses full-length Transmembrane protein 109 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMEM (TMEM109 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51856-plasmid-map-sequence-id-79743

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  5. pENTR_L1-Turbo-NES-YFP-STOP-L2

    Plasmid
    #127358
    Purpose
    gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127358-plasmid-map-sequence-id-246304

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  6. MMP11-bio-His

    Plasmid
    #53408
    Purpose
    Expresses full-length Stromelysin-3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    MMP11 (MMP11 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53408-plasmid-map-sequence-id-83189

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  8. VWF-bio-His

    Plasmid
    #53419
    Purpose
    Expresses full-length von Willebrand factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    VWF (VWF Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53419-plasmid-map-sequence-id-83209

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  13. pJG367

    Plasmid
    #91185
    Purpose
    BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    nAtCas9_H840A+gNt_R2+gNt_F2+donor
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91185-plasmid-map-sequence-id-182419

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  14. pJG382

    Plasmid
    #91189
    Purpose
    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    nAtCas9_D10A+gNt_R2+gNt_F2+donor
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91189-plasmid-map-sequence-id-182420

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  15. pFastBacDual with LFn + Needle

    Plasmid
    #84868
    Purpose
    Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Needle
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84868-plasmid-map-sequence-id-162901

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  18. pMOD_B2515b

    Plasmid
    #91073
    Purpose
    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    AtU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91073-plasmid-map-sequence-id-181900

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  19. mKate2-P2A-APEX2-OSBP

    Plasmid
    #67664
    Purpose
    Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    mKate2-P2A-APEX2-OSBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67664-plasmid-map-sequence-id-117189

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Showing: 7241 - 7260 of 10634 results