Showing: 7241 - 7260 of 20566 results
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Plasmid#61857PurposeExpresses a gRNA plasmid targeting the prion gene's exon 3, positive strandDepositorInsertPrP guiding sequence (Prnp Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterhU6 promoterAvailabilityAcademic Institutions and Nonprofits only -
pAC149-pCR8-dCas9VP160
Plasmid#48221PurposedCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP160ExpressionMutationD10A;H840A nuclease-deficientPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAC94-pmax-dCas9VP160-2A-puro
Plasmid#48226PurposedCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
UseCRISPRTags2A-puro, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pAC95-pmax-dCas9VP160-2A-neo
Plasmid#48227PurposedCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
UseCRISPRTags2A-neo, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
MSP1101
Plasmid#65773PurposeHuman expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, and T1337R mutations in C…PromoterCMV & T7AvailabilityAcademic Institutions and Nonprofits only -
MSP2440
Plasmid#72250PurposeHuman expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
MSP2443
Plasmid#72252PurposeHuman expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP
Plasmid#64216PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFPExpressionMammalianMutationPromoterCBh and U6AvailabilityAcademic Institutions and Nonprofits only -
pY003 (pFnCpf1_delta Cas)
Plasmid#69974PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes
UseCRISPRTagsExpressionBacterialMutationCas genes are deleted from locusPromoterAvailabilityAcademic Institutions and Nonprofits only -
pY002 (pFnCpf1_min)
Plasmid#69975PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes nd artificial promoter
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X
Plasmid#128112PurposeAcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9DepositorInsertAcrX (AcrIIC1 N3F/D15Q/A48I)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1-mCherry chimera #10
Plasmid#128113PurposeExpresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9DepositorInsertAcrIIC1-mCherry chimera #10
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XS)Cas9
Plasmid#127382PurposeExpresses Cas9 at very high levelDepositorInsertuORF-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
UAS-u(S)Cas9
Plasmid#127383PurposeExpresses Cas9 at high levelDepositorInsertuORF(S)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailabilityAcademic Institutions and Nonprofits only -
UAS-u(M)Cas9
Plasmid#127384PurposeExpresses Cas9 at medium levelDepositorInsertuORF(M)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
UAS-u(L)Cas9
Plasmid#127385PurposeExpresses Cas9 at low levelDepositorInsertuORF(L)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XL)Cas9
Plasmid#127386PurposeExpresses Cas9 at very low levelDepositorInsertuORF(XL)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XXL)Cas9
Plasmid#127387PurposeExpresses Cas9 at very low levelDepositorInsertuORF(XXL)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
UAS-FRT-GFP-FRT-u(M)Cas9
Plasmid#127388PurposeCas9 inducible by removal of a GFP flip-out cassetteDepositorInsertFRT-GFP-FRT-uORF(M)Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-P2A-EGFP (RTW4177)
Plasmid#139988PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only
Showing: 7241 - 7260 of 20566 results