Addgene

We narrowed to 28,062 results for: eng

Showing: 7221 - 7240 of 28062 results

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pADS

Plasmid

#19040

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

codon-optimized synthetic amorphadiene synthase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCS4092

Plasmid

#131741

Purpose

Theophylline switch Theo-CAUAA

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

Theophylline switch Theo-CAUAA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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p6xEGFP-N1

Plasmid

#122166

Purpose

Expression of 6xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

6xEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pG1AK-mCherry

Plasmid

#71737

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

mCherry

Use

Synthetic Biology; Shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

pRplsWT

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ132D

Plasmid

#69288

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

Plasmid

#169326

Purpose

Expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC myc hPOT1 delta OB

Plasmid

#13241

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 delta OB (POT1 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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InterCatch-GFP

Plasmid

#186900

Purpose

Cell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-sfGFP-SpyCatcher003-sfCherry1-10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-Citrine (525)

Plasmid

#70718

Purpose

Expression vector for Citrine purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ131D

Plasmid

#69287

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1259

Plasmid

#61251

Purpose

Cas9 plasmid with sgRNA deleted; for use in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

Deletion

Use

CRISPR

Tags

Expression

Worm

Mutation

Deleted sgRNA(F+E) sequence from pJW1219

Promoter

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pNC-KSE401

Plasmid

#193435

Purpose

Nimble cloning-compatible vector for genome editing in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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CAG::PSAML141F,Y115F:GlyR-IRES-GFP

Plasmid

#32480

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F:GlyR

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pGD431

Plasmid

#132382

Purpose

contains the sequence of mRuby3for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mRuby3

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMT Draper-mCherry

Plasmid

#126667

Purpose

Inducible expression of Draper-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper mCherry

Use

Tags

Expression

Insect

Mutation

C813Y- see depositor comments below

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pND004

Plasmid

#200950

Purpose

MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAL-pyk_NT

Plasmid

#74072

Purpose

pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pyk (NT)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3-mRuby3

Plasmid

#203491

Purpose

jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al Elife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Gateway entry vector

Tags

His-tag and mRuby3

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-A21

Plasmid

#90318

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS A21

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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