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150,386 results

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Rc/CMV cyclin E

Plasmid

#8963

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin E (CCNE1 Human)

Use

Tags

GPI linker

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pKM491-Flag-His

Plasmid

#109282

Purpose

ORBIT integrating plasmid for C-terminal tagging with FLAG-His tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Flag-His

Use

Orbit integrating plasmid

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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PAP-delta10-cHIS

Plasmid

#21716

Depositor

Andrew Bohm

Article

Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

Insert

polyA polymerase (PAP1 Budding Yeast)

Use

Tags

HIS

Expression

Bacterial

Mutation

30 amino acids off the c terminus

Promoter

Available since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only
CXCR2-Tango

Plasmid

#66260

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CXCR2 (CXCR2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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1036 pcDNA3 Myr HA Akt1

Plasmid

#9008

Purpose

Mammalian expression of human Akt1 with myr, HA tags

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Tags

HA and myr

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pCDF-GFPplus

Plasmid

#172718

Purpose

Expression of a fast folding GFP variant GFP plus

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

GFP plus

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV_PUUV_GP

Plasmid

#207301

Purpose

Mammalian expression of Puumala virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

PUUV_GP

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLX208 CMV sTurboID (C)-HA-FRB-ERM

Plasmid

#153007

Purpose

Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C) (G74)-ERM (Cb5)

Use

Lentiviral

Tags

FRB and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDest-565

Plasmid

#11520

Purpose

Gateway destination vector for bacterial expression; His,GST tag

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway destination vector

Tags

His6-GST

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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GRK2 S670A

Plasmid

#45755

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 S670A (GRK2 Bovine)

Use

Tags

Expression

Mammalian

Mutation

S670A

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
mBFP-APP-mGFP

Plasmid

#196694

Purpose

Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Use

Tags

mEGFP and mtagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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SIRT3 Flag

Plasmid

#13814

Purpose

Mammalian expression of human SIRT3 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT3 (SIRT3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

Plasmid

#51094

Purpose

Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore

Depositor

Jonathan Ting

Insert

oChIEF(E163A/T199C)

Use

AAV

Tags

P2A-dTomato

Expression

Mammalian

Mutation

E163A/T199C

Promoter

EF1a

Available since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1

Plasmid

#35535

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

Plasmid

#213024

Purpose

Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

Depositor

Martin S. Taylor, Oliver Weichenrieder

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

LINE-1 ORF2p (238-1061)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

T7 / Lac

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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KCNQ4-Myc

Plasmid

#197329

Purpose

Plasmid expressing an antigen targeting the KCNQ4/Kv7/4 K+ channel subunit

Depositor

Thomas Jespersen

Insert

KCNQ4 (KCNQ4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-TVA-eGFP-N2cG

Plasmid

#175439

Purpose

Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

PcUBI4-2

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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alpha Synuclein-VC

Plasmid

#89471

Purpose

expresses human alpha Synuclein fused to the C-terminal part of Venus

Depositor

Bradley Hyman, Tiago Outeiro

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Use

Tags

C-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-LC3B WT

Plasmid

#123114

Purpose

Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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