-
Plasmid#200136PurposeType 3a part for Modular Cloning in YeastDepositorInsertIMS-Mia40 (2-70)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-nLuc
Plasmid#212611PurposeFor expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.DepositorInsertTornado-CVB3-nLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP_rescue_construct
Plasmid#190641PurposePuromycin-selectable expression of GFP in Drosophila S2 cellsDepositorInsertEGFP
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-mutTornado-split-nLuc
Plasmid#212707PurposeTornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.DepositorInsertmutTornado-CVB3-split-nLuc
UseTagsExpressionMutationDeleted the partial 3'ribozyme sequence in T…PromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-Rab5a
Plasmid#72901PurposeEarly endosome-targeted mCherry-tagged recruiter constructDepositorInsertRab5a (RAB5A Human)
UseLentiviralTagsFKBP(F36V) and mCherryExpressionMammalianMutationfragment a.a. 2-215PromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
BiP-mGFP P495L
Plasmid#62232PurposeMutant of hamster BiP-mGFP unable to bind clients.DepositorInserthamster BiP-mGFP-KDEL
UseTagsmGFPExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-Rab11a
Plasmid#72902PurposeRecycling endosome-targeted mCherry-tagged recruiter constructDepositorInsertRab11a (RAB11A Human)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationfragment a.a. 2-216PromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-mCherry-sfGFP
Plasmid#213860PurposeFor Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertmCherry-sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterCaMV 35SAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCW008
Plasmid#83526Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha2
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN3
Plasmid#79257PurposeExpresses EGFP-tagged DmPAN3 in S2 cellsDepositorInsertPAN3 (CG11486 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2
Plasmid#79256PurposeExpresses EGFP-tagged DmPAN2 in S2 cellsDepositorInsertDmPAN2 (PAN2 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector
Plasmid#114430PurposeTarget a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 cassette to the mouse TIGRE locusDepositorInsertGCaMP6s, tdTomato, tTA2
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterTRE2, CAGAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai139 (TIT2L-GFP-ICL-TPT) targeting vector
Plasmid#114426PurposeTarget a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locusDepositorInsertGFP, tdTomato, tTA2,
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterTRE2, CAGAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai167 (TIT2L-ChrimsonR-tdT-ICL-tTA2) targeting vector
Plasmid#114431PurposeTarget a Cre-dependent ChrimsonR-tdTomato cassette and a tTA2 cassette into the mouse TIGRE locusDepositorInsertChrimsonR-tdTomato, tTA2
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterTRE2, CAGAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai170 (TIT2L-ASAP2s-Kv-ICL-tTA2) targeting vector
Plasmid#114435PurposeTarget a Cre-dependent voltage sensor ASAP2s cassette into the mouse TIGRE locusDepositorInsertASAP2s-Kv, tTA2
UseCre/Lox and Mouse TargetingTagsKv2.1 tagExpressionMutationPromoterTRE2, CAGAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseTagsExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
GOSR2-eGFP
Plasmid#209886PurposeExpresses human GOSR2 (GS27) fused to eGFP in mammalian cellsDepositorInsertGOSR2 (GOSR2 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
GOSR2-mCherry
Plasmid#209885PurposeExpresses human GOSR2 (GS27) fused to mChery in mammalian cellsDepositorInsertGOSR2 (GOSR2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 YFP
Plasmid#84911Purposeexpression of TDP-43 YFP in mammalian cellsDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only