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Plasmid#202091PurposeReceiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pRegev_ExonSkip_Backbone
Plasmid#204187PurposeBackbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).DepositorInsertFirst exon and intron for beta globin splicing minigene
UseTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-AsCas12j-2
Plasmid#191655PurposeAll-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2DepositorInsertAsCas12j-2
UseTagsExpressionBacterialMutationPromoterrpsL(XC)-BbsIAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux LuxI
Plasmid#193627PurposeInducible expression of LuxI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LuxI is codon optimized for E. coli.DepositorInsertLuxI
UseTags6xHisExpressionBacterialMutationPromoterLux promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6-mNeonC
Plasmid#178461PurposeThe mNeonGreen fluorescent protein with an N-terminal 6xHis and a C-terminal free-Cysteine in pET28aDepositorInsertmNeonGreen
UseTags6xHisExpressionBacterialMutationFree cysteine at the C-terminusPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6-2xmNeonC
Plasmid#178463PurposeA tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.DepositorInsertmNeonGreen dimer
UseTags6xHisExpressionBacterialMutationFree cysteine at the C-terminus of the dimerPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6_fold-Z _Gogy
Plasmid#201828PurposeExpresses H6_fold-Z _Gogy in bacterial cellsDepositorInsertH6_fold-Z _Gogy
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ef1a-DIO-eGCaMP(plus)
Plasmid#201149PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(plus)DepositorInserteGCaMP+
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Gal4-degFusionRed-p65
Plasmid#199464PurposeExpression of the synthetic photodegradable transcription factor Gal4-degFusionRed-p65 in mammalian cells.DepositorInsertGal4-degFusionRed-p65
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
H7_fold-K _Mussoc
Plasmid#201830PurposeExpresses H7_fold-K _Mussoc in bacterial cellsDepositorInsertH7_fold-K _Mussoc
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6_fold-U _Nomur
Plasmid#201829PurposeExpresses H6_fold-U _Nomur in bacterial cellsDepositorInsertH6_fold-U _Nomur
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6_fold-C _Rei
Plasmid#201827PurposeExpresses H6_fold-C _Rei in bacterial cellsDepositorInsertH6_fold-C _Rei
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
H5_fold-0 _Chantal
Plasmid#201826PurposeExpresses H5_fold-0_Chantal in bacterial cellsDepositorInsertH5_fold-0 _Chantal
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc
Plasmid#199759PurposeElongation reporter construct to quantify elongation duration of 4_CGA stallingDepositorInsertsynYFP[TTG/AGA]-4xArg[CGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB302-er-mCherry
Plasmid#186287PurposeBinary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)DepositorInserter-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067B
Plasmid#200252PurposepBac-U6-gRNA(doublesex)-3xp3-tdTomatoDepositorInsert4 gRNAs targeting Doublesex
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc
Plasmid#199760PurposeElongation reporter construct to quantify elongation duration of 6_CGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[CGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc
Plasmid#199761PurposeElongation reporter construct to quantify elongation duration of 6_AGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[AGA]-cLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_WT_KDEL_pBABEpu
Plasmid#183688PurposeRetroviral expression of ER targeted H6-tagged Halotag Wild typeDepositorInsertER Halo
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_aa1-133-HiBiT
Plasmid#198313PurposeLoads a truncated payload; for identification of Pdp1 packaging domainDepositorInsertPdp1_aa1-133-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only