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Showing: 7181 - 7200 of 27793 results
  1. pHL 1032

    Plasmid
    #52997
    Purpose
    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Inserts
    RhyB sRNA
    ompC
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pHL 762

    Plasmid
    #52990
    Purpose
    pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Inserts
    MicC sRNA
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    −56 to +141 bp and −81 to +36 bp
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pQZ210

    Plasmid
    #106414
    Purpose
    Co-expresses trans SNARE complex with pDC183 in E.coli
    Depositor
    Inserts
    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Synthetic, Rat)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Fragment 191-256 , Codon optimization and Fragmen…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pDC183

    Plasmid
    #106415
    Purpose
    Co-expresses trans SNARE complex with pQZ210 in E. coli
    Depositor
    Inserts
    SNAP25 fragment 141-204 (Snap25 Synthetic, Rat)
    SNAP25 Fragment 7-83 (Snap25 Synthetic, Rat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fragment 141-204, Codon optimization and Fragment…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pGEM-GFP-URA3-GFP

    Plasmid
    #72606
    Purpose
    Template for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeast
    Depositor
    Inserts
    GFP (full)
    URA3
    GFP (partial)
    Optional Linker
    Use
    Tags
    Expression
    Mutation
    Includes full 819 bp coding sequence of URA3, 435…
    Promoter
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3.1.Gαq-LgB97

    Plasmid
    #134360
    Purpose
    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.
    Depositor
    Insert
    Gαq-LgB97 (GNAQ Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 7181 - 7200 of 27793 results