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AiP1713 - pAAV-AiE2405m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214526

Purpose

AiE2405m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv, 2024.06.10.597244

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1334 - pAAV-AiE2116m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214485

Purpose

AiE2116m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv, 2024.06.10.597244

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1492 - pAAV-AiE2004m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214501

Purpose

AiE2004m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv, 2024.06.10.597244

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1332 - pAAV-AiE2114m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214483

Purpose

AiE2114m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv, 2024.06.10.597244

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV102

Plasmid

#215308

Purpose

Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

PTac

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV103

Plasmid

#215309

Purpose

Expresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV104

Plasmid

#215310

Purpose

Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

PAraBAD

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-UBC-MCP-mNeonGreen

Plasmid

#207581

Purpose

The lentiviral plasmids for expression of MCP-NeonGreen

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

MS2 coat protein mNeonGreen fusion

Use

Lentiviral

Tags

mNeonGreen

Expression

Mutation

Promoter

UBC

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_LTB4R

Plasmid

#208087

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R (LTB4R Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

none

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196079

Purpose

Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196085

Purpose

Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-dsarm(E)

Plasmid

#187870

Purpose

Gal4/UAS expression of dSarm(isoE)

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

dSarm isoform E (Sarm Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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ADAM10_Halo_C_allele

Plasmid

#178151

Purpose

Donor vector for endogenous tagging of human ADAM10 at the C-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (ADAM10 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-GAPDH-IRES-EGFP

Plasmid

#115918

Purpose

donor to insert IRES-EGFP at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-EGFP

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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MP-K13

Plasmid

#69657

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA polB

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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MP-P7

Plasmid

#69666

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR polB

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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Klf2-t2A-mCherry

Plasmid

#127539

Purpose

Expresses mouse Klf2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Klf2 (Klf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-hCas3

Plasmid

#134920

Purpose

Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas3 with bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Lifeact-Halo

Plasmid

#193328

Purpose

mammalian transient expression - expression of Lifeact-Halo

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

Lifeact

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rif

Plasmid

#23233

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rif (RHOF Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only