Addgene

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p63(1-29)-mCherry-N1

Plasmid

#67895

Purpose

Expresses of fusion of mCherry with the N-terminal 29 amino acids of p63RhoGEF

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

p63RhoGEF(1-29)-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMXLK_dFq

Plasmid

#114237

Purpose

POC12377, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-hphMX6

Plasmid

#105161

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-hphMX6

Use

Tags

GBP-RFP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMXLK_pFa

Plasmid

#114229

Purpose

POC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLC_pFa

Plasmid

#114219

Purpose

POC12355, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-DH

Plasmid

#67898

Purpose

Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

mCherry-DH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1-DH

Plasmid

#67901

Purpose

Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

mCherry-FKBP12-DH

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMXLP_pFa

Plasmid

#114239

Purpose

POC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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POC1468

Plasmid

#213756

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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POC1467

Plasmid

#213755

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-RBP-WDR5

Plasmid

#209702

Purpose

Mammalian expression of WDR5 with N terminal RBP fusion

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

RBP-WDR5

Use

Tags

RBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-Clover

Plasmid

#209721

Purpose

Mammalian expression of clover localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-Clover

Use

Tags

Cos4

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5

Plasmid

#209706

Purpose

Mammalian expression of y-ATOM biosensor for WDR5

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_SH2

Plasmid

#209708

Purpose

Mammalian expression of y-ATOM biosensor for SH2

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_SH2

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-SH2

Plasmid

#209717

Purpose

Mammalian expression of SH2 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-SH2

Use

Tags

Cos4

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-NbGFP_miRFP670nano3

Plasmid

#209719

Purpose

Mammalian expression of NbGFP-miRFP670nano3 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-NbGFP_miRFP670nano3

Use

Tags

Cos4

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1aKpn1

Plasmid

#203307

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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lacI-Ptac-lacO-IMBB4-pUC

Plasmid

#170770

Purpose

test plasmid for 5RM assembly, expresses lacI repressor

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

lacI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

lacI promoter

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMXLP_eFq

Plasmid

#114248

Purpose

POC12390, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type e-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBC_pFa

Plasmid

#114249

Purpose

POC12391, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge