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Plasmid#42333PurposeThis plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationPromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 U6-SaDMDR7-U6-SaDMDL2
Plasmid#78603PurposeAAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23DepositorInsertU6-SaDMDR7-U6-SaDMDL2
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 SV40-CMV-SaCas9-3xNLS
Plasmid#78601PurposeAAV vector containing SaCas9DepositorInsertSV40-CMV-SaCas9-3xNLS
UseAAV and CRISPRTagsHA tagExpressionMammalianMutationPromoterSV40, CMV promotersAvailable sinceDec. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-PGD2-1.1
Plasmid#214764PurposeAAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoterDepositorInsertGRAB-PGD2-1.1 sensor
UseAAVTagsExpressionMutationPromoterEF-1-alpha promoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 U6-SaAi9L-U6-SaAi9R
Plasmid#78604PurposeAAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassetteDepositorInsertgRNAs for SaCas9 targeting Ai9 locus
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo U6-SpAi9L-U6SpDMDL
Plasmid#78608PurposeExpresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22DepositorInsertU6-SpAi9L-U6SpDMDL
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo U6-SpAi9R-U6-SpDMDR
Plasmid#78609PurposeExpresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23DepositorInsertU6-SpAi9R-U6-SpDMDR
UseUnspecifiedTagsExpressionMutationPromoterU6Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-PGD2-1.1-IRES-mcherry
Plasmid#214763PurposeExpress GRAB-PGD2-1.1 sensor and membrane tethered mCherry under CMV promoterDepositorInsertGRAB-PGD2-1.1 sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP
Plasmid#207025PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371A I375A D384A Y387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Pho8-406
Plasmid#207006PurposeIntegration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.DepositorInsertPHO8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Osh1-406
Plasmid#207007PurposeIntegration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.DepositorInsertOSH1
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-PGD2-1.0-IRES-mcherry
Plasmid#214762PurposeExpress GRAB-PGD2-1.0 sensor and membrane tethered mCherry under CMV promoterDepositorInsertGRAB-PGD2-1.0 sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15
Plasmid#207050PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationP85L P86LPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15
Plasmid#207051PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationT56A R65A T87APromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15
Plasmid#207052PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationR65A P85A P86APromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15
Plasmid#207053PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationR65A P85L P86LPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15
Plasmid#207054PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationL55W T56A V61W R65APromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-eYFP (AAV PHP.eB)
Viral Prep#117382-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-NLS-GFP (AAV PHP.eB)
Viral Prep#104061-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only