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Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK Mma PylT (CUA)
Plasmid#154782Purposeplasmid with single copy of Mma PylT (CUA anticodon)DepositorInsertMma PylT
UseTagsExpressionMammalianMutationU25CPromoterh7SKAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-codon-modified HTK
Plasmid#34563DepositorInsertcodon-modified HTK
UseTagsExpressionBacterialMutationcodon modified HTK using mouse codon usage tablePromoterpUC57 bacteria promoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98976PurposeCas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(N863A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19 ACMV DNA-B 1.5mer
Plasmid#159135PurposeInfectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-BDepositorInsertACMV DNA-B 1.5mer
UseInfectious clone for bombarding plantsTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top-Toehold-mCherry
Plasmid#87318PurposeExpresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.DepositorInsertSplit-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK Mma PylT (UUA)
Plasmid#154783Purposeplasmid with single copy of Mma PylT (UUA anticodon)DepositorInsertMma PylT
UseTagsExpressionMammalianMutationU25C and UUA anticodonPromoterh7SKAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(1004)
Plasmid#44365DepositorInsertUAS1B16-TEF(1004) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(1004)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-dirTopo-P2A-aGFP
Plasmid#61974PurposeA directional cloning vector of PCR-amplified cDNAs for mammalian expressionDepositorInsertslacZ alpha
EGFP
UseTagsExpressionMammalianMutationIt contains silent mutationsPromoterAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Sa sgRNA expression vector
Plasmid#107720PurposeFor in vitro transcription of Sa sgRNA from the T7 promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~Bottom-T
Plasmid#87315PurposeControl plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system (promoter control)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 delta gap
Plasmid#49091PurposeTemplate for generating gene 60 mRNA, coding gap deletionDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationCoding gap and coding gap STOP have been deleted.PromoterT7Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ30
Plasmid#65028PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneUseTagslacZExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC 3GLA UAS HAi
Plasmid#71763Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tagDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(1004)
Plasmid#44366DepositorInsertUAS1B8-TEF(1004) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(1004)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
Mishler2014-No-aptamer-controlluciferase-pUC19
Plasmid#63856PurposeThis pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromotertacAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(203)
Plasmid#44378DepositorInsertUAS1B8-TEF(203) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(203)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-UAS1B8-TEF(804)
Plasmid#44368DepositorInsertUAS1B8-TEF(804) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(804)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUCmini-NFKB1-PGK-EGFP
Plasmid#204018PurposeHomologous donor template for NFKB1 knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB1-PGK-tdTomato
Plasmid#204019PurposeHomologous donor template for NFKB1 knockout expressing tdTomatoDepositorInserttdTomato
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only