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  5. pcDNA3.1.Gα12-LgB115

    Plasmid
    #134363
    Purpose
    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα12-LgB115 (GNA12 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134363-plasmid-map-sequence-id-306384

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  6. pcDNA3.1-mouse cdk6 wt

    Plasmid
    #75170
    Purpose
    Mammalian expression of mouse cdk6
    Depositor
    Martine Roussel
    Insert
    cyclin dependent kinase 6 (Cdk6 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pcDNA3.1-Nanoluc-ccdB

    Plasmid
    #87070
    Purpose
    Mammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87070-plasmid-map-sequence-id-190070

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  17. pcDNA3.1(+)-AKAP95-FRB

    Plasmid
    #138254
    Purpose
    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    AKAP95-FRB (AKAP8 Human)
    Use
    Tags
    FRB rapamycin binding domain and HA epitope
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    138254-plasmid-map-sequence-id-307498

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Showing: 701 - 720 of 5023 results