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Plasmid#159418PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coliDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbmCherry
Plasmid#184676PurposeNIR-Fb to mCherryDepositorInsertNIR-FbmCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
BbZIP-cALFA
Plasmid#160498PurposeFor the protein expression of BbZIP with ALFA tag at C-terminus in E. coliDepositorInsertBbZIP
UseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDX_init containing Sb-loop
Plasmid#132697PurposepDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curveDepositorInsertSb_loop
UseM13 phagmid vectorTagsExpressionMutationPromoterlacAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTSG
Plasmid#159420PurposeTransient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cellsDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-EGFP
Plasmid#108896PurposeMultisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertAPEX2-P2A-EGFP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFPmG1
Plasmid#50444PurposeExpression of a EGFP variant in mammalian cellsDepositorInsertGFPmG1
UseTagsExpressionMammalianMutationE143K, N147QPromoterCAGAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME-EGFP-P2A-APEX2-NS
Plasmid#108895PurposeMultisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertEGFP-P2A-APEX2
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceMay 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Cre
Plasmid#50797PurposeExpression of Cre recombinase under the control of the UAS-promoterDepositorInsertCre recombinase
UseMulti-speciesTagsExpressionMutationPromoterUAS-hsp70Available sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB2AR-miRFP670nano3
Plasmid#184671PurposeBeta-2-adrenergic receptor internally tagged with miRFP670nano3DepositorInsertB2AR-miRFP670nano3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTSG
Plasmid#159419PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiaeDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionYeastMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb2E7
Plasmid#184681PurposeNIR-Fb to HIV gp41DepositorInsertNIR-Fb2E7
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
UseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTSG
Plasmid#162389PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cellDepositorInsertthermostable GFP (TGP)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb4EIG
Plasmid#184683PurposeNIR-Fb to E.coli dihydrofolate reductaseDepositorInsertNIR-Fb4EIG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb59H10
Plasmid#184682PurposeNIR-Fb to HIV p24DepositorInsertNIR-Fb59H10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Gly42TAG
Plasmid#125265PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Gly42 nanobody
UseTagsHis-tagExpressionBacterialMutationPromotertrc promoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-ppiB
Plasmid#202482PurposeExpresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletionDepositorInsertpeptidyl-prolyl cis-trans isomerase (ppiB)
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-GFPuv
Plasmid#202463PurposeExpresses GFPuv with T7 inductionDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-LCAmp-trxB
Plasmid#202465PurposeExpresses a guide RNA to silence trxB after phosphate depletionDepositorInserttrxB guide RNA
UseTagsExpressionBacterialMutationPromoterugpBAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only