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Plasmid#91060PurposeModule B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK285 (Stag-3FLAG-Bsr)
Plasmid#72801PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Bsr
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2517
Plasmid#91086PurposeModule C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_26H
Plasmid#91150PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:barDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2301
Plasmid#91067PurposeModule B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG367
Plasmid#91185PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_H840A+gNt_R2+gNt_F2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG382
Plasmid#91189PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_D10A+gNt_R2+gNt_F2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-hPOLISc
Plasmid#60819Purposeto express human Pol iota recoded to yeast codons in yeastDepositorInsertPOLI (POLI Human)
UseTagsc-mycExpressionYeastMutationoptimized to yeast codon usagePromoterGAL1Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SPORT6-RNF114-RINGmut
Plasmid#69922PurposeExpresses mutant myc-RNF114 in mammalian cellsDepositorInsertRNF114 (RNF114 Human)
UseTagsExpressionMammalianMutationC29A, C32APromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2309
Plasmid#91069PurposeModule B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRTagsExpressionMutationPromoterYAOAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21B
Plasmid#91129PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_26G
Plasmid#91149PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:barDepositorInsertEngineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25G
Plasmid#91144PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SPORT6-RNF114-UIMmut
Plasmid#69923PurposeExpresses mutant myc-RNF114 in mammalian cellsDepositorInsertRNF114 (RNF114 Human)
UseTagsExpressionMammalianMutationS224PPromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23D
Plasmid#91141PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25I
Plasmid#91146PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2121
Plasmid#91065PurposeModule B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoterGmUbiAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2516
Plasmid#91084PurposeModule C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterAt7SLAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22D
Plasmid#91136PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21D
Plasmid#91131PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only