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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

Plasmid

#159657

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-R

Plasmid

#122345

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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RBM4_2xRRM-ZNF_pGEX

Plasmid

#135154

Purpose

Recombinant expression of RBM4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM4 (RBM4 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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PCBP4_3xKH_pGEX

Plasmid

#135156

Purpose

Recombinant expression of PCBP4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PCBP4 (PCBP4 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTV-mHdac1-tdiRFP670-PEST2

Plasmid

#122351

Purpose

Targeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Hdac1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha-Y206S

Plasmid

#136435

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Y206S (disrupts PDGF binding, but still binds HCM…

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha-V242K

Plasmid

#136436

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

V242K (disrupts PDGF binding, but still binds HCM…

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Met-Eif4a1-tdiRFP670-PEST

Plasmid

#122371

Purpose

Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST-2A with homology arms for Eif4a1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-L

Plasmid

#122346

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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UNK_6xZNF_pGEX

Plasmid

#215703

Purpose

Recombinant expression of UNK with dual-affinity tag

Depositor

Christopher Burge, Daniel Dominguez

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

UNK (UNK Human)

Use

Tags

GST and SBP

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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Msi1_2xRRM_pGEX

Plasmid

#215704

Purpose

Recombinant expression of mouse Msi1 with dual-affinity tag

Depositor

Christopher Burge, Daniel Dominguez

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

Musashi-1 (Msi1 Mouse)

Use

Tags

GST and SBP

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-wt-HaloTag

Plasmid

#201551

Purpose

Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1 (partial)

Use

Tags

HaloTag

Expression

Insect

Mutation

Codon-optimized for Drosophila expression

Promoter

Ac5

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p202-Syt1-C2A-wt

Plasmid

#201553

Purpose

Expresses wild-type Octopus synaptotagmin-1 C2A domain in E. coli

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Synaptotagmin-1 C2A domain (partial)

Use

Tags

Expression

Bacterial

Mutation

Codon-optimized for E. coli

Promoter

RBS

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pCAGGS

Plasmid

#206052

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pcDNA3

Plasmid

#206051

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS pcDNA3

Plasmid

#206057

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-IEo

Plasmid

#201992

Purpose

AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Depositor

Wei Xu

Article

Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

Insert

Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Use

AAV

Tags

Expression

Mutation

Promoter

Tight TRE

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-NeoCOVGT3-d2EGFP

Plasmid

#201446

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT3_d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT1-d2EGFP

Plasmid

#201447

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT1_d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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