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Showing: 701 - 720 of 12305 results

  4. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

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  6. pRA1SEGFPTcIfm

    Plasmid
    #193777
    Purpose
    Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PR promoter and PLTetO-1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193777-plasmid-map-sequence-id-389829

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  15. ATP1A1_G3_EGFP_Nick_Dual_sgRNA

    Plasmid
    #178095
    Purpose
    Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + EGFP nick sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178095-plasmid-map-sequence-id-350125

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  19. SIN40C.EFS.eGFP.pre

    Plasmid
    #169276
    Purpose
    Constitutive overexpression of GFP under the control of EFS promoter; for miRNA 3'UTR binding assays.
    Depositor
    Dirk Heckl
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169276-plasmid-map-sequence-id-337928

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  20. Nup54_mEGFP494(-6)

    Plasmid
    #163434
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 6 amino acids) at amino acid position 494
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163434-plasmid-map-sequence-id-321676

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Showing: 701 - 720 of 12305 results