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Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertGEM-GECO
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXPR_047
Plasmid#107145Purposemeasures Cas9 activityDepositorInsertGFP + sgRNA for GFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dR8.2 dvpr
Plasmid#8455Purpose2nd generation lentiviral packaging plasmid. Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mScarlet-I
Plasmid#98816PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorArticleInsert3xNLS
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3550
Plasmid#145026PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTTP1B
Plasmid#91723Purposeintegration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNADepositorTypeEmpty backboneUseIntegration-proficient plasmid vectors for mycoba…TagsExpressionMutationPromoterAvailable sinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLARRY-EGFP
Plasmid#140025PurposeLentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNADepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightR (AAV9)
Viral Prep#187180-AAV9PurposeReady-to-use AAV9 particles produced from pAAV_hSyn1_nLightR (#187180). In addition to the viral particles, you will also receive purified pAAV_hSyn1_nLightR plasmid DNA. Syn-driven expression of red norepinephrine sensor nLightR. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhuman Synapsin-1TagsNoneAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmcherry α-catenin WT
Plasmid#178646Purposeexpress wild-type α-cateninDepositorInsertα-catenin (CTNNA1 Human)
UseTagsmcherryExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCutamp
Plasmid#140632PurposePlasmid-curing in Escherichia coli by targeting the AmpR promoterDepositorInsertSpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
VCP(wt)-EGFP
Plasmid#23971DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationnonePromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-Lamin-A
Plasmid#206027PurposeExpresses GFP-tagged Lamin A in mammalian cellsDepositorInsertLamin-A (LMNA Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV5)
Viral Prep#20298-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
3HA-ERHBD-Hoxb8
Plasmid#212932PurposeExpresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell linesDepositorUseRetroviralTagsTriple HA tagExpressionMammalianMutationPromoterMSCV LTRAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAD48-daf-2 RNAi
Plasmid#34834DepositorInsertdaf-2 (daf-2 Nematode)
UseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P2
Plasmid#125035PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertPDC1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 neo
Plasmid#104996PurposeThis lentiviral construct delivers hSpCas9 and G418 resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_Her2-BFP_RHL027
Plasmid#164823PurposeLentiviral vector for constitutive expression of HER2+TM(23-675)-BFPDepositorInsertExtracellular and transmembrane region of ERBB2 (ERBB2 Human)
UseLentiviralTagstagBFPExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG (AAV9)
Viral Prep#187179-AAV9PurposeReady-to-use AAV9 particles produced from pAAV_hSyn1_nLightG (#187179). In addition to the viral particles, you will also receive purified pAAV_hSyn1_nLightG plasmid DNA. Synapsin-driven expression of norepinephrine sensor nLightG. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhuman Synapsin-1TagsNoneAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)
Viral Prep#100798-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only