Addgene

We narrowed to 10,634 results for: GAN

Showing: 7081 - 7100 of 10634 results

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FAB(PX-LD)

Plasmid

#214426

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FKBP-V5

Use

Tags

CFAST-Halo and FKBP-V5-RspAN

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(WT)

Plasmid

#206406

Purpose

Mammalian expression of hARL1(WT)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

Plasmid

#198337

Purpose

PB plasmid expressing Halo-tagged PP7 coat protein

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

PP7 coat protein 3x Halo-GB1

Use

Piggybac

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_HNF4G_Hormone-recep

Plasmid

#109916

Purpose

Protein expression and purification of HNF4G_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HNF4G_Hormone-recep (HNF4G Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag ERbeta

Plasmid

#35562

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only

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pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(P691S)-FLAG

Plasmid

#164973

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P691S

Promoter

EF1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR2

Plasmid

#161753

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPB CAG Otx2-IRES-EGFP

Plasmid

#153947

Purpose

piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Otx2 (Otx2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-mir-17-92

Plasmid

#64100

Purpose

expresses mir-17-92

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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NPHS-bio-His

Plasmid

#53334

Purpose

Expresses full-length Nephrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

NPHS (NPHS1 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#194703

Purpose

Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Tags

APOBEC1-HA-P2A-mRuby

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-333

Plasmid

#104194

Purpose

Expresses human AR aa 1-333 in pGEX-5x-1 bacteral expression vector with GST

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-333 (AR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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GST-AR-174-566

Plasmid

#104197

Purpose

Expresses human AR-174-566 in pGEX-3x bacterial expression vector as fusion protein with GST

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 174-566 (AR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-VPS4A

Plasmid

#116924

Purpose

expresses mEGFP-VPS4A in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

VPS4A (VPS4A Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

silent mutation in position 4059 C to T

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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THBS1-bio-His

Plasmid

#53417

Purpose

Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

THBS1 (THBS1 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

Plasmid

#174890

Purpose

Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

U25C PylT

Promoter

EF1

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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