Addgene

We narrowed to 18,518 results for: omp

Showing: 7061 - 7080 of 18518 results

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pT7-EGFP-C1-HsNot1

Plasmid

#37370

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot1 (CNOT1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-EYFP

Plasmid

#104052

Purpose

An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.V1

Insert

EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CAAX PuroR

Plasmid

#166228

Purpose

Lentiviral plasmid with Puromycin selection for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

mCherry-CAAX

Use

Lentiviral

Tags

CAAX

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSL0341 (pTarget)

Plasmid

#200906

Purpose

Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

pTarget

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ACE2-GFP

Plasmid

#154962

Purpose

pcDNA3.1-ACE2-GFP

Depositor

Utpal Pajvani

Article

Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

Insert

ACE2-GFP (ACE2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-renilla-luciferase-beta-globin-control

Plasmid

#184393

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Use

Tags

6xHis and NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)

Plasmid

#184394

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globin

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSL2783 (pTnsC-VP64_Vch)

Plasmid

#200896

Purpose

Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsC-VP64

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2669 (pTnsABf_Vch)

Plasmid

#200897

Purpose

Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsA-TnsB

Use

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAT007

Plasmid

#171634

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mTagBFP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH056

Plasmid

#171637

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-tdTomato
mTagBFP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-4x

Plasmid

#124678

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBS mouse c-Myc

Plasmid

#14972

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (Myc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Nucleotides 2578-3294

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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MMLV-RT(dRH) for Split-PE (pEH50)

Plasmid

#190106

Purpose

Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT(delta RNAseH)

Use

Tags

bpNLS

Expression

Mammalian

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2

Plasmid

#25031

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP2 (DCP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-WIP

Plasmid

#11772

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP (WIPF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only