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Plasmid#35946DepositorInsertLRRK2 (LRRK2 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
LADL Anchor
Plasmid#127664PurposeEncodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoterDepositorInsertdCas9-CIBN-2A-Puro
UseTags3XFLAGExpressionMammalianMutationCas9 (D10A,H840A) mutantPromoterEF1aAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_LacZ_sgRNA
Plasmid#74179Purposelentiviral vector expressing sgRNA targeting LacZDepositorInsertLacZ sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc-y1sgRNA-Cas9
Plasmid#49331PurposeExpresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertsUseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgDHODH-4
Plasmid#186022Purposeknock out DHODH in mammalian cellsDepositorInsertDihydroorotate Dehydrogenase (Quinone) (DHODH Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP18 (CK2alpha' K69M)
Plasmid#27090DepositorInsertCK2alpha' K69M (CSNK2A2 Human)
UseTagsHAExpressionMammalianMutationK69MPromoterCMVAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2
Plasmid#182359PurposePositive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.DepositorInsertUltra-pan-neuronal positive control pUNP::gfp(NLS)
UseTagsExpressionWormMutationPromoterPromoter Ultra Pan NeuronalAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB07 (CK2beta)
Plasmid#27091DepositorInsertCK2beta (CSNK2B Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_Luciferase_sgRNA
Plasmid#74190Purposelentiviral vector expressing sgRNA targeting LuciferaseDepositorInsertLuciferase sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS R71/75E-HA
Plasmid#130919PurposeExpresses human cGAS-HA with R71/75E point mutation; Puromycin selection markerDepositorInserthuman cGAS R71/75E
UseTagsHAExpressionMammalianMutationaa 71 and 75 mutated to glutamatePromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
SHIP2 WT pBABE-His-Xpress
Plasmid#126584PurposeRetroviral infection of MEF cells (puromycin selection)DepositorInsertSHIP2 (INPPL1 Human)
UseRetroviralTagsHis Xpress (N-terminal)ExpressionMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemTagsExpressionMammalianMutationY64L (mutRFP)PromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S87A
Plasmid#36056DepositorInsertalpha-synuclein S87A (SNCA Human)
UseAAVTagsExpressionMammalianMutationS87APromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21c-His-Xa-Gluc-Xa-C9D-TGinsert
Plasmid#124660Purposereporter protein which emits bright blue fluorescenceDepositorInsertGaussia luciferase-C9D-TGinsert
UseTagsIEGRTMGDDDGDDDGDDD(FactorXa,TMG,C9D-Tag) and MHHH…ExpressionBacterialMutationchanged glutamic acid 100 to alanine, and glycine…PromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-ASAP2s
Plasmid#101275PurposeGenetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIDepositorInsertASAP2s
UseAAVTagsExpressionMammalianMutationPromoterCAMKIIaAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_PSMD1_sgRNA_5
Plasmid#74181Purposelentiviral vector expressing sgRNA targeting PSMD1DepositorInsertPSMD1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)
Plasmid#184334PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorInsertNrxn3-alpha (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_PSMD1_sgRNA_1
Plasmid#74180Purposelentiviral vector expressing sgRNA targeting PSMD1DepositorInsertPSMD1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV:: HA-hM4Dnrxn
Plasmid#52525PurposeExpresses N-terminal HA tagged hM4DnrxnDepositorInsertHA-hM4Dnrxn (CHRM4 Human, Synthetic)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_EIF3D_sgRNA_1
Plasmid#74187Purposelentiviral vector expressing sgRNA targeting EIF3DDepositorInsertEIF3D sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only