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Plasmid#229454PurposeExpresses IraM with His-tagDepositorInsertIraM
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUB_mCherry-Rluc100
Plasmid#210600PurposeExpresses mCherry-Optimal RenillaDepositorInsertmCherry-Optimal Renilla
UseLentiviral and LuciferaseTagsmCherryExpressionMammalianMutationPromoterUBC humanAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
JD180
Plasmid#229486PurposeAdenoviral helper plasmid containing inducible E2A and L4-22k/33k genesDepositorInsertE2A, L4-22k/33k, mCitrine
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterTre (E2A, L4-22k/33k), ETR (mCitrine)Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
JD179
Plasmid#229496PurposeAdenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I geneDepositorInsertE2A, L4-22k/33k, VA RNA I
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterTre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
JD226
Plasmid#229484PurposeAdenoviral helper plasmid containing E2A, VA RNA I and L4-22k genesDepositorInsertE2A, VA RNA I, L4-22k
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterCMV (E2A), Ef1a (L4-22k) and wt promoter (VA RNA …Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseA-6xHis
Plasmid#214808PurposeExpression of 6xhis-tagged micrococcal nuclease A (MNaseA)DepositorInsertOmpA-Micrococcal nuclease A
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGA016
Plasmid#228231PurposeLevel 0 for 5xOp (GreenGate A module)DepositorInsert5xOp
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-mScarlet-i
Plasmid#191196PurposeFusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & mScarlet-I fusion
UseTags6xHis and mScarlet-IExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmGFP-TEV-mScarlet-i
Plasmid#191199PurposeFusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & mScarlet-I fusion
UseTags6xHis and mScarlet-IExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGC018
Plasmid#228232PurposeLevel 0 for LhG4 (GreenGate C module)DepositorInsertGR-LhG4
UseSynthetic BiologyTagsExpressionMutationPromoter-Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-CRISPRi
Plasmid#221136PurposeCoxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.DepositorInsert3xF-dCas9
UseCRISPRTags3xFLAGExpressionBacterialMutationPromotercbu1169 promoterAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH72
Plasmid#202487PurposeExpresses the nanobody VHH72 with T7 inductionDepositorInsertnanobody VHH72
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH-C9BoNT-A
Plasmid#202490PurposeExpresses the nanobody C9 BoNT/A with T7 inductionDepositorInsertnanobody C9 BoNT/A
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-GFPuv
Plasmid#202463PurposeExpresses GFPuv with T7 inductionDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_UENU_donor
Plasmid#221459PurposeVector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.DepositorInsert3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_reporter_vector
Plasmid#221460PurposeVector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.DepositorInsertgypsy insulator-EGFP-neutral spacer DNA-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dm_λHA-FLuc100_CSC
Plasmid#210592PurposeExpresses lambdaHA-optimal Firefly (CSC metric; Drosophila)DepositorInsertLambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)
UseTagslambda HAExpressionInsectMutationPromoterAc5Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dm_Opt_Renilla_CSC
Plasmid#210593PurposeExpresses Optimal Renilla luciferase (CSC metric; Drosophila)DepositorInsertOptimal Renilla luciferase (Optimized for Drosophila, CSC metric)
UseTagsExpressionInsectMutationPromoterAc5Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_OptλHAramp-FLuc100
Plasmid#210577PurposeExpresses Optimal lambda HA-Optimal Firefly luciferaseDepositorInsertOptimal lambda HA-Optimal Firefly luciferase
UseTagslambda HAExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only