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Plasmid#131336PurposegRNA to delete control region near HoxD. Use with HoxD del control usDepositorInsertCTRL-gRNA-C-del
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HoxD del us1
Plasmid#131337PurposegRNA to delete a nucleation site near HoxD. Use with HoxD del ds1DepositorInsertEVX2-gRNA-N-del
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HoxD del control us
Plasmid#131335PurposegRNA to delete control region near HoxD. Use with HoxD del control dsDepositorInsertCTRL-gRNA-N-del
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
EED-KO-gRNA-1
Plasmid#131321PurposegRNA to knockout EED. Use with EED-KO-gRNA-2DepositorInsertEED-KO-gRNA-1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS22
Plasmid#129710PurposeExpresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusionDepositorInsertEscherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
UseTags10-Histidine tag cleavable with PreScission Prote…ExpressionBacterialMutationRssB E134G:E135S:E136G:E137SPromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG
Plasmid#127325PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG
Plasmid#127324PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG
Plasmid#127327PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG
Plasmid#127326PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL251 GG sgRNA
Plasmid#126436PurposeTo target a protospacer with GG at the cut site.DepositorInsertspacer against human genome with GG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL510 CA sgRNA
Plasmid#126430PurposeTo target a protospacer with CA at the cut site.DepositorInsertspacer against human genome with CA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL401 GC sgRNA
Plasmid#126435PurposeTo target a protospacer with GC at the cut site.DepositorInsertspacer against human genome with GC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL071 AG sgRNA
Plasmid#126428PurposeTo target a protospacer with AG at the cut site.DepositorInsertspacer against human genome with AG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL402 AC sgRNA
Plasmid#126427PurposeTo target a protospacer with AC at the cut site.DepositorInsertspacer against human genome with AC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL507 GA sgRNA
Plasmid#126434PurposeTo target a protospacer with GA at the cut site.DepositorInsertspacer against human genome with GA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL512 CG sgRNA
Plasmid#126432PurposeTo target a protospacer with CG at the cut site.DepositorInsertspacer against human genome with CG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL063 GT sgRNA
Plasmid#126437PurposeTo target a protospacer with GT at the cut site.DepositorInsertspacer against human genome with GT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL516 CT sgRNA
Plasmid#126433PurposeTo target a protospacer with CT at the cut site.DepositorInsertspacer against human genome with CT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL515 AA sgRNA
Plasmid#126426PurposeTo target a protospacer with AA at the cut site.DepositorInsertspacer against human genome with AA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL511 CC sgRNA
Plasmid#126431PurposeTo target a protospacer with CC at the cut site.DepositorInsertspacer against human genome with CC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only