Addgene

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pSCL.102

Plasmid

#184987

Purpose

Express -Eco1 RT-P2A-Cas9

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-P2A-Cas9

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.005

Plasmid

#184971

Purpose

Express Cas9 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Integrating inducible cassette: Cas9

Use

Tags

SV40NLS

Expression

Yeast

Mutation

Promoter

Gal1-10

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET-GFP-9S [rpoA]

Plasmid

#176221

Purpose

Expression of GFP-9S [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

GFP-9S [rpoA]

Use

Synthetic Biology

Tags

His-GFP

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi28TAG31TAG

Plasmid

#188996

Purpose

Express chimadanin with TAG at 28 and 31 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 28 and 31position

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi31TAG

Plasmid

#188985

Purpose

Express chimadanin with TAG at 31 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 31 position

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_NT135

Plasmid

#183121

Purpose

pLentiGuide modified to express mCherry and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-TOMM20-Halo

Plasmid

#129348

Purpose

Halo Tag, Mitochondrial outer membrane localization

Depositor

Yasushi Okada

Article

Wang et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

Halo (Promega)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Cdc42

Plasmid

#180582

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm1_A

Plasmid

#145886

Purpose

Insect Expression of Dm-Lsm1

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

Dm-Lsm1

Use

Tags

Expression

Insect

Mutation

Two silent mutation compared to the sequence give…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm4_A

Plasmid

#145889

Purpose

Insect Expression of Dm-Lsm4

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

Dm-Lsm4

Use

Tags

Expression

Insect

Mutation

one non silent mutation G127R compared to the seq…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm7_A

Plasmid

#145891

Purpose

Insect Expression of DmLSm7

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm7 (LSm7 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAraQCas-BsaI

Plasmid

#170633

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm3_A

Plasmid

#145888

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-GgVCL/*TBM

Plasmid

#162790

Purpose

MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

Depositor

Laura Lafon-Hughes

Article

et al PeerJ

Insert

Mutated Gallus gallus vinculin (G454V) (VCL Chicken)

Use

Tags

IRES-GFP

Expression

Bacterial and Mammalian

Mutation

changed Gly 454 to Val

Promoter

beta actin

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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Adeno-miR-23b

Plasmid

#112538

Purpose

Adenovirus overexpression

Depositor

Shusheng Wang

Article

Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

Insert

miR-23b (MIR23B Human)

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-17

Plasmid

#160717

Purpose

TrpC Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites

Use

Synthetic Biology

Tags

Expression

Mutation

Incorporation of BsaI restriction sites

Promoter

Available since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-15

Plasmid

#160715

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-FLAG peptide

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-3

Plasmid

#160703

Purpose

tef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tef1-alpha

Use

Synthetic Biology

Tags

Expression

Mutation

Incorporation of BsaI restriction sites

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-2

Plasmid

#160702

Purpose

gpdh promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

gpdh

Use

Synthetic Biology

Tags

Expression

Mutation

Incorporation of BsaI restriction sites

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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