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Plasmid#52422Purposemammalian expression of flag tagged rat CLC3 short transcriptDepositorInsertCLC3 (Clcn3 Rat)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 S103L (#624)
Plasmid#99942Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationS103LPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)
Plasmid#168190PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.DepositorInsertHA-nLag6-(EAAAK)4-OGA(544-706)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-spa-GCaMP6f
Plasmid#67563Purposebacterial expression of a photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseTagsHIS-tagExpressionBacterialMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ914
Plasmid#44489DepositorInsertMinimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
UseGateway expression cloneTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-103)
Plasmid#60357PurposeExpress the RRM1 of mouse MSI1 in bacterial cells for protein purificationDepositorInsertmouse MSI1 (7-103) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains amino acids 7-103 of Msi1PromoterT7Available sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3 humanN-Flag SadBS
Plasmid#66881PurposeMammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadBDepositorInsertSadB (BRSK1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense
Plasmid#46839PurposeExpress cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translatedDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
II801: pMVP (L5-L4) ecDHFR DD
Plasmid#121721PurposepMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.DepositorInsertecDHFR Degron Domain (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7g2
Plasmid#14625DepositorInsertMKK7g2 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PAiRFP2
Plasmid#44271DepositorInsertPAiRFP2
UseTagsExpressionBacterialMutationK69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…PromoterpBADAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-Craf SSDD
Plasmid#118337PurposeExpression of human CrafDepositorInsertCraf SSDD (RAF1 Human)
UseTagsFlagExpressionMammalianMutationSSDDPromoterCMVAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEnt R3L2 TetO(sh) mCh-Rs1-2
Plasmid#24417DepositorInsertRs1
UseEntry vectorTagsFLAG and mCherryExpressionMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG484E-myc
Plasmid#107471PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG484EPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS477F-myc
Plasmid#107470PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS477FPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO mutant ITR
Plasmid#67905PurposePiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutationDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-ACC1*
Plasmid#126740Purposeyeast plasmid overexpressing ACC1 from Komagataella phaffiiDepositorInsertacc1*
UseTagsExpressionYeastMutationT3452GPromoterGAPAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RLuc8C-FHA1-NES
Plasmid#138210PurposeEncodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.DepositorInsertRLuc8C-FHA1-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKAsub-RLuc8N-NES
Plasmid#138211PurposeEncodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKAsub-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only