Addgene

We narrowed to 13,025 results for: Mpl;

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SJZ9-amdS

Plasmid

#228261

Purpose

Template plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

amdS

Use

Tags

Expression

Yeast

Mutation

Promoter

ENO2 S.c.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ7-KAN

Plasmid

#228260

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

kan

Use

Tags

Expression

Yeast

Mutation

Promoter

GPM1 S.c.

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ4-NAT

Plasmid

#228257

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

nat1

Use

Tags

Expression

Yeast

Mutation

Promoter

FBA1 S.c.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-N)

Plasmid

#187084

Purpose

Medium expression of Complex I with subunit N-HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Use

Tags

HA tag nuoN

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAMBIA-FLS2prom::FLS2-3xmyc

Plasmid

#86158

Purpose

FLS2 expression

Depositor

Silke Robatzek

Article

Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

3xmyc

Expression

Plant

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCR-Blunt_hM3O

Plasmid

#112638

Purpose

Plasmid for PCR amplification of hM3O DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

hM3O

Use

Pcr template

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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MKT074

Plasmid

#231997

Purpose

MicF RNA toehold switch with NanoLuc complementation peptide reporter

Depositor

Melissa Takahashi

Article

Diaz et al MicroPubl Biol. 2025 Jan 2;2025:10.17912

Insert

MicF RNA toehold switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ5-HPH

Plasmid

#228258

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

hph

Use

Tags

Expression

Yeast

Mutation

Promoter

SSA1 S.c.

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ2-TRP1

Plasmid

#228256

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

TRP1

Use

Tags

Expression

Yeast

Mutation

Promoter

BBC1 S.c.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ10-LEU2

Plasmid

#228262

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

LEU2

Use

Tags

Expression

Yeast

Mutation

Promoter

LEU2 K.l.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-H*HA-N)

Plasmid

#187086

Purpose

Medium expression of Complex I with subunits N and H -HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Use

Tags

HA tags nuoN, nuoH

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCR-Blunt_mWasabi

Plasmid

#112640

Purpose

Plasmid for PCR amplification of mWasabi DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

mWasabi

Use

Pcr template

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW-H1 empty vector

Plasmid

#25870

Purpose

3rd generation lentiviral vector

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pCAMBIA-FLS2prom::FLS2-3xmyc-GFP

Plasmid

#86157

Purpose

FLS2-GFP expresssion

Depositor

Silke Robatzek

Article

Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

3xmyc and GFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pES133

Plasmid

#127552

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Insert

F5-flanked tetracycline resistance cassette

Use

Tags

Expression

Bacterial

Mutation

Multiple SNPs in tetA (originally from pBR322) to…

Promoter

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 delta gap

Plasmid

#49091

Purpose

Template for generating gene 60 mRNA, coding gap deletion

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Coding gap and coding gap STOP have been deleted.

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCEF-S15-FRB-Bla1

Plasmid

#190916

Purpose

Protein fragment complementation

Depositor

Jakob Reiser

Article

Ou et al Gene Ther. 2013 Jan;20(1):43-50. doi: 10

Insert

S15-FRB-Bla1 fusion protein

Use

Tags

N-terminal fragment of BLA fused to c-Src and FRB…

Expression

Mammalian

Mutation

Promoter

CEF

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 to gap

Plasmid

#49158

Purpose

Template for generating gene 60 mRNA, sequence 5' of coding gap only

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Coding gap and downstream sequence have been del…

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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BJ5183 cells

Bacterial Strain

#16398

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
BA14

Bacterial Strain

#186997

Purpose

Deletion of the nuo operon

Depositor

Steven Vik

Article

Amarneh et al Biochim Biophys Acta. 2006 Dec;1757(12):

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only