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Plasmid#39292DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pnxABE-flgn
Plasmid#187932PurposeA-to-G base editorDepositorInsertecTadA(8e)-nxCas9 (D10A)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
CyTERM-mGFP
Plasmid#62237PurposeER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.DepositorInsertCyTERM-mGFP
UseTagsmGFPExpressionMammalianMutationsignal anchor of rabbit cytochrome p450 and monom…PromoterCMVAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Plac-dCas9 (Plac)
Plasmid#105235PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosaDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599APromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP2463
Plasmid#107774PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pBBR1-mcs5
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBJZ_001
Plasmid#155357PurposeGentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRF0G-IodoY
Plasmid#113645PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.DepositorInsertIodoYRS and tRNA gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEN_TT 3xFLAG Luciferase
Plasmid#98391PurposeGateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tagDepositorInsertLuciferase
UseEntry vector for gateway cloningTags3xFLAGExpressionMutationPromoterTRE tight and TRE tight promoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pFBD-SpyTag-noro-VLP
Plasmid#165989PurposeExpresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteinsDepositorInsertSpyTag-noro-VLP (ORF2 norovirus, Synthetic)
UseTagsSpyTagExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
ClCry4 PHR
Plasmid#160552PurposeExpression of protein for various experiments including finding interaction partnersDepositorInsertClcry4 HR
UseTagsHexa hisExpressionInsectMutationPromoterAvailable sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB227
Plasmid#68618PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-LIC-Bse
Plasmid#26108PurposeSGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis6 - TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT85_SpdCas9_pmcDA1
Plasmid#204614PurposeCRISPR cytosine deaminase integrative plasmid for Mycoplasma bovisDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA-gRic6T
Plasmid#106401PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoterpj23119Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Arp2/3_pBIG2abcd
Plasmid#173683PurposeInsect expression of the 7-subunit Human Arp2/3 complexDepositorInsertsArp2
Arp3
ArpC2
ArpC3-FLAG
ArpC4
ArpC5
ArpC1
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
SeeSaw Reporter (SSR1.0)
Plasmid#50392PurposeSSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.DepositorInsertSSR1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB202
Plasmid#68593PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only