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Plasmid#121981Purposefor generating kank3 riboprobes for WISH in zebrafishDepositorInsertkank3 (kank3 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL6-V5-His6
Plasmid#67422PurposeExpression of hArl6 with C-term V5 and His6 tag in E. coliDepositorInsertARL6 (ARL6 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL8B-no stop
Plasmid#67335PurposehArl8b without stop codon in Gateway Entry vectorDepositorInsertARL8B (ARL8B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL6-no stop
Plasmid#67329PurposehArl6 without stop codon in Gateway Entry vectorDepositorInsertARL6 (ARL6 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL11-no stop
Plasmid#67341PurposehArl11 without stop codon in Gateway Entry vectorDepositorInsertARL11 (ARL11 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 442 T to C; aa C148RPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL6-V5-His6
Plasmid#67443PurposeMammalian expression of hArl6 with C-term V5 and His6 tagDepositorInsertARL6 (ARL6 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL1-no stop
Plasmid#67317PurposehArl1 without stop codon in Gateway Entry vectorDepositorInsertARL1 (ARL1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 231 C to T; silent mutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eBE-S1
Plasmid#101092PurposeExpresses eBE-S1 in mammalian cellsDepositorInserteBE-S1
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL4C-V5-His6
Plasmid#67423PurposeExpression of hArl4c with C-term V5 and His6 tag in E. coliDepositorInsertARL7 (ARL4C Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL11
Plasmid#67340PurposehArl11 with stop codon in Gateway Entry vectorDepositorInsertARL11 (ARL11 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 261 G to A; silent mutation; bp 442 T to C; aa…PromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV cleavable His10ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#173208PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N mScarlet-I-TOSI donor
UseCRISPRTagsExpressionMammalianMutationPromoterhPGK1Available sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-MAFG-V5-miniTurbo
Plasmid#207818PurposeC-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertMAFG-V5-miniTurbo
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-MAFG
Plasmid#207817PurposeN-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-MAFG
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-A
Plasmid#207823PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-B
Plasmid#207824PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-ZNF746-C571A
Plasmid#207826PurposeDox-inducible expression of ZNF746-C571ADepositorInsertZNF746-C571A (ZNF746 Human)
UseLentiviralTagsExpressionMutationC571APromoterTREAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo G226A E321K ORF29
Plasmid#200026PurposeLentiviral vector for expression of KSHV ORF29 with G226A and E321K mutationDepositorInsertORF29
UseLentiviralTagsExpressionMutationGlycine 226 changed to alanine, glutamic acid 321…PromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiT-mEmerald-MED1 donor
Plasmid#197867PurposeDonor plasmid used for building mEmerald-MED1 knock-in mESCsDepositorInsertCRSP210 (Med1 Mouse)
UseMouse TargetingTagsmEmeraldExpressionMutationPromoterNAAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTij_sg_mmu_Med6_C_terminal
Plasmid#197869PurposeCRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only