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Plasmid#224484PurposeGateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)DepositorInsertV5-mTagBFP2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-V5-mTagBFP2 (JDW 871)
Plasmid#224531PurposeA Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2DepositorInsertV5-mTagBFP2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-dCas9-BFP-KRAB
Plasmid#221502PurposeCRISPRi machinery fish codon optimizedDepositorInsertdCas9-tagBFP-KRAB
UseFish expressionTagsExpressionMutationD10A and H839A point mutationsPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mTagBFP2 rat DJ-1 shRNA
Plasmid#222870PurposeExpresses mTagBFP2 along with an shRNA against rat DJ-1DepositorInsertDJ-1 shRNA (Park7 Rat)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail
Plasmid#220079PurposeSAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue FluorophoreDepositorInsertSACM1l-C389S-Fis1tail (SACM1L Human)
UseTagsExpressionMammalianMutationC389SdeltaTMDPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO mTagBFP2-GABARAP
Plasmid#212110PurposeExpression of N-terminally tagged GABARAP with mTagBFP2DepositorInsertmTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-H2BmTagBFP2-oG
Plasmid#217976PurposeAAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G proteinDepositorInsertsH2BmTagBFP2
oG
UseAAVTagsmTAGBFP2ExpressionMutationPromoterhSynAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(CDK6-E1(6))-PGKpuro2ABFP-W
Plasmid#200487PurposeLentiviral vector expressing gRNA targeting human CDK6-E1DepositorInsertCDK6-E1(6) (CDK6 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(CXCR4-E1(15))-PGKpuro2ABFP-W
Plasmid#200478PurposeLentiviral vector expressing gRNA targeting human CXCR4-E1DepositorInsertCXCR4-E1(15) (CXCR4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(CXCR4-E2(38))-PGKpuro2ABFP-W
Plasmid#200481PurposeLentiviral vector expressing gRNA targeting human CXCR4-E2DepositorInsertCXCR4-E2(38) (CXCR4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-FRB-Rab5
Plasmid#214272PurposeMammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker)DepositorArticleInsertmTagBFP2-FRB-Rab5A (RAB5A Canis lupus familiaris (Dog))
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-EBFP2-OptoSTIM1(CRY2clust)
Plasmid#184716PurposeOptoSTIM1(CRY2clust)DepositorInsertOptoSTIM1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ARID2(13))-PGKpuro2ABFP-W
Plasmid#200471PurposeLentiviral vector expressing gRNA targeting human ARID2DepositorInsertARID2(13) (ARID2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(CXCR4-E3(48))-PGKpuro2ABFP-W
Plasmid#200483PurposeLentiviral vector expressing gRNA targeting human CXCR4-E3DepositorInsertCXCR4-E3(48) (CXCR4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BRD9(13))-PGKpuro2ABFP-W
Plasmid#200473PurposeLentiviral vector expressing gRNA targeting human BRD9DepositorInsertBRD9(13) (BRD9 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RHOA(15))-PGKpuro2ABFP-W
Plasmid#200493PurposeLentiviral vector expressing gRNA targeting human RHOADepositorInsertRHOA(15) (RHOA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZNF90_5-5)-PGKpuroBFP-W
Plasmid#212003PurposeExpress gRNA against ZNF90 with puro and BFPDepositorInsertsgRNA targeting ZNF90 (ZNF90 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(VENTX_5-5)-PGKpuroBFP-W
Plasmid#211993PurposeExpress gRNA against VENTX with puro and BFPDepositorInsertsgRNA targeting VENTX (VENTX Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZIC2_5-4)-PGKpuroBFP-W
Plasmid#211994PurposeExpress gRNA against ZIC2 with puro and BFPDepositorInsertsgRNA targeting ZIC2 (ZIC2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZIC2_5-5)-PGKpuroBFP-W
Plasmid#211995PurposeExpress gRNA against ZIC2 with puro and BFPDepositorInsertsgRNA targeting ZIC2 (ZIC2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only