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Plasmid#121980Purposefor generating kank2 riboprobes for WISH in zebrafishDepositorInsertkank2 (LOC571549 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6/ERGIC2-WT
Plasmid#51479PurposeExpresses ERGIC2-WT in mammalian cellsDepositorInsertERGIC2-WT (ERGIC2 Human)
UseTagsnoneExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ914
Plasmid#44489DepositorInsertMinimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
UseGateway expression cloneTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
BB1_23_eGFP
Plasmid#98518PurposeBB1 with insert: CDS eGFPDepositorInserteGFP
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP1 OR sfGFP
Plasmid#107364PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressedDepositorInsertOR gate + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPi
Plasmid#127548PurposeEmpty control for pGAPi-based RNAi silencing in tandem with APT transcript segmentDepositorInsertAdenine Phosphorybosyltransferase transcript segment
UseRNAi; Empty controlTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Act5C-pDONR221
Plasmid#124242PurposeGateway entry clone of Drosophila Actin5C cDNA.DepositorInsertDrosophila Actin 5C (Act5C Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB3aK_14*
Plasmid#98529Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pLAT1
Plasmid#98509PurposeBB1 with insert: promoter PLAT1DepositorInsertLAT1 promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pPFK300
Plasmid#98510PurposeBB1 with insert: promoter PPpPfkDepositorInsertPpPfk promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pDAS2
Plasmid#98513PurposeBB1 with insert: promoter PDAS2DepositorInsertDAS2 promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
MCV-HF LTS220A/S239A
Plasmid#112191PurposeMerkel cell polyomavirus molecular cloneDepositorInsertMCV-HF LTS220A/S239A
UseTagsExpressionMutationT to G mutations at 3313 (nt) and 3370 (nt) of fu…PromoterNone from vector. Insert contains native promoter…Available sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-DGKA
Plasmid#31294DepositorInsertdiacylglycerol kinase, alpha (DGKA Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pRPL2A
Plasmid#98508PurposeBB1 with insert: promoter PRPL2ADepositorInsertRPL2A promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
kank4_pJet1.2
Plasmid#121982Purposefor generating kank4 riboprobes for WISH in zebrafishDepositorInsertkank4 (kank4 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rae1-pDONR221
Plasmid#124240PurposeGateway entry clone of Drosophila Rae1 cDNA.DepositorInsertDrosophila Rae1 (Rae1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDA_103
Plasmid#133460PurposeH1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.DepositorInsertcontrol guide
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pDAS1
Plasmid#98515PurposeBB1 with insert: promoter PDAS1DepositorInsertDAS1 promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK015
Plasmid#105347PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only